Genetically targeted all-optical electrophysiology with a transgenic cre-dependent optopatch mouse

Shan Lou, Yoav Adam, Eli N. Weinstein, Erika Williams, Katherine Williams, Vicente Parot, Nikita Kavokine, Stephen Liberles, Linda Madisen, Hongkui Zeng, Adam E. Cohen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

Recent advances in optogenetics have enabled simultaneous optical perturbation and optical readout of membrane potential in diverse cell types. Here, we develop and characterize a Cre-dependent transgenic Optopatch2 mouse line that we call Floxopatch. The animals expressed a blue-shifted channelrhodopsin, CheRiff, and a near infrared Archaerhodopsin-derived voltage indicator, QuasAr2, via targeted knock-in at the rosa26 locus. In Optopatch-expressing animals, we tested for overall health, genetically targeted expression, and function of the optogenetic components. In offspring of Floxopatch mice crossed with a variety of Cre driver lines, we observed spontaneous and optically evoked activity in vitro in acute brain slices and in vivo in somatosensory ganglia. Cell-type-specific expression allowed classification and characterization of neuronal subtypes based on their firing patterns. The Floxopatch mouse line is a useful tool for fast and sensitive characterization of neural activity in genetically specified cell types in intact tissue.

Original languageAmerican English
Pages (from-to)11059-11073
Number of pages15
JournalJournal of Neuroscience
Volume36
Issue number43
DOIs
StatePublished - 26 Oct 2016
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2016 the authors.

Keywords

  • Optogenetics
  • Optopatch
  • Transgenic mice
  • Voltage imaging

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