Genome-wide demethylation of Arabidopsis endosperm

Tzung Fu Hsieh, Christian A. Ibarra, Pedro Silva, Assaf Zemach, Leor Eshed-Williams, Robert L. Fischer, Daniel Zilberman

Research output: Contribution to journalArticlepeer-review

541 Scopus citations

Abstract

Parent-of-origin-specific (imprinted) gene expression is regulated in Arabidopsis thaliana endosperm by cytosine demethylation of the maternal genome mediated by the DNA glycosylase DEMETER, but the extent of the methylation changes is not known. Here, we show that virtually the entire endosperm genome is demethylated, coupled with extensive local non-CG hypermethylation of small interfering RNA-targeted sequences. Mutation of DEMETER partially restores endosperm CG methylation to levels found in other tissues, indicating that CG demethylation is specific to maternal sequences. Endosperm demethylation is accompanied by CHH hypermethylation of embryo transposable elements. Our findings demonstrate extensive reconfiguration of the endosperm methylation landscape that likely reinforces transposon silencing in the embryo.

Original languageAmerican English
Pages (from-to)1451-1454
Number of pages4
JournalScience
Volume324
Issue number5933
DOIs
StatePublished - 12 Jun 2009
Externally publishedYes

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