TY - JOUR
T1 - Genome-wide dissection of Fusarium resistance in tomato reveals multiple complex loci
AU - Sela-Buurlage, M. B.
AU - Budai-Hadrian, O.
AU - Pan, Q.
AU - Carmel-Goren, L.
AU - Vunsch, R.
AU - Zamir, D.
AU - Fluhr, R.
PY - 2001
Y1 - 2001
N2 - Resistance to different pathogenic races of Fusarium oxysporum f. sp. lycopersici (F. o. lycopersici) was explored at two genomic levels in tomato. Six independent Fusarium resistance loci were identified by comparing the responses of a complete set of 53 lines carrying different introgressed regions of the Lycopersicon pennellii genome in a L. esculentum background. The loci confer varying degrees of resistance to different races of the pathogen. Corresponding map positions from different tomato species were aligned and in some cases revealed parallel resistance to F. o. lycopersici with qualitative changes in race specificities. One of the loci identified corresponds to the previously characterized complex resistance locus I2, which is involved in resistance to F. o. lycopersici race 2. A novel member of this locus, I2C-5, which belongs to the NBS-LRR family of resistance genes, was cloned and shown to confer partial resistance in transgenic plants. Thus, at a particular complex locus gene members can confer full or partial resistance to F. o. lycopersici race 2. The results of our whole-genome mapping analysis underline the robust independent origin of resistance to a particular disease and demonstrate the conservation of resistance features at syntenic loci, together with the rapid diversification of genes for innate resistance within loci.
AB - Resistance to different pathogenic races of Fusarium oxysporum f. sp. lycopersici (F. o. lycopersici) was explored at two genomic levels in tomato. Six independent Fusarium resistance loci were identified by comparing the responses of a complete set of 53 lines carrying different introgressed regions of the Lycopersicon pennellii genome in a L. esculentum background. The loci confer varying degrees of resistance to different races of the pathogen. Corresponding map positions from different tomato species were aligned and in some cases revealed parallel resistance to F. o. lycopersici with qualitative changes in race specificities. One of the loci identified corresponds to the previously characterized complex resistance locus I2, which is involved in resistance to F. o. lycopersici race 2. A novel member of this locus, I2C-5, which belongs to the NBS-LRR family of resistance genes, was cloned and shown to confer partial resistance in transgenic plants. Thus, at a particular complex locus gene members can confer full or partial resistance to F. o. lycopersici race 2. The results of our whole-genome mapping analysis underline the robust independent origin of resistance to a particular disease and demonstrate the conservation of resistance features at syntenic loci, together with the rapid diversification of genes for innate resistance within loci.
KW - Durable resistance
KW - NBS-LRR
KW - Oligogenic resistance
KW - Resistance gene evolution
UR - http://www.scopus.com/inward/record.url?scp=0034923079&partnerID=8YFLogxK
U2 - 10.1007/s004380100509
DO - 10.1007/s004380100509
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C2 - 11523783
AN - SCOPUS:0034923079
SN - 1617-4615
VL - 265
SP - 1104
EP - 1111
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
IS - 6
ER -
