Abstract
The family Erythrobacteraceae, belonging to the order Sphingomonadales, class Alphaproteobacteria, is globally distributed in various environments. Currently, this family consist of seven genera: Altererythrobacter, Croceibacterium, Croceicoccus, Erythro-bacter, Erythromicrobium, Porphyrobacter and Qipengyuania. As more species are identified, the taxonomic status of the family Erythrobacteraceae should be revised at the genomic level because of its polyphyletic nature evident from 16S rRNA gene sequence analysis. Phylogenomic reconstruction based on 288 single-copy orthologous clusters led to the identification of three separate clades. Pairwise comparisons of average nucleotide identity, average amino acid identity (AAI), percentage of conserved protein and evolutionary distance indicated that AAI and evolutionary distance had the highest correlation. Thresholds for genera boundaries were proposed as 70% and 0.4 for AAI and evolutionary distance, respectively. Based on the phylo-genomic and genomic similarity analysis, the three clades were classified into 16 genera, including 11 novel ones, for which the names Alteraurantiacibacter, Altericroceibacterium, Alteriqipengyuania, Alteripontixanthobacter, Aurantiacibacter, Paraurantiaci-bacter, Parerythrobacter, Parapontixanthobacter, Pelagerythrobacter, Tsuneonella and Pontixanthobacter are proposed. We reclas-sified all species of Erythromicrobium and Porphyrobacter as species of Erythrobacter. This study is the first genomic-based study of the family Erythrobacteraceae, and will contribute to further insights into the evolution of this family.
| Original language | American English |
|---|---|
| Pages (from-to) | 4470-4495 |
| Number of pages | 26 |
| Journal | International Journal of Systematic and Evolutionary Microbiology |
| Volume | 70 |
| Issue number | 8 |
| DOIs | |
| State | Published - 2020 |
Bibliographical note
Funding Information:information This study was funded by the National Natural Science Foundation of China (No. 31770004 and No. 91851114), the Natural Science Foundation of Zhejiang Province (No. LR17D060001), the National Program for Support of Top-Notch Young Professionals. Acknowledgements Aeb. aerius 100921-2T, Aeb. buctensis M0322T, Aeb. luteolus SW-109T, Aeb. marinus H32T, Aeb. xixiisoli S36T and Erb. arachoides RC4-10-4T were generously donated by Professor Yong Li (Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry), Professor Jie Lv (Beijing University of Chemical Tech-nology), Professor Jung-Hong Yoon (Korea Research Institute of Bioscience and Biotechnology), Professor Zongze Shao (Third Institute of Oceanography, Ministry of Natural Resources), Professor Zhil-iang Yu (Zhejiang University of Technology) and Professor Yongqin Liu (Institute of Tibetan Plateau Research, Chinese Academy of Sciences), respectively.
Funding Information:
This study was funded by the National Natural Science Foundation of China (No. 31770004 and No. 91851114), the Natural Science Foundation of Zhejiang Province (No. LR17D060001), the National Program for Support of Top-Notch Young Professionals.
Publisher Copyright:
© 2020, Microbiology Society. All rights reserved.
Keywords
- AAI
- Erythrobacteraceae
- Evolutionary distance
- Phylogenomic reconstruction