Genomic gain of 5p15 leads to over-expression of Misu (NSUN2) in breast cancer

Michaela Frye; Ilaria Dragoni; Suet Feung Chin; Immaculada Spiteri; Agata Kurowski; Elena Provenzano; Andrew Green; Ian O. Ellis; Donna Grimmer; Andrew Teschendorff; Christos C. Zouboulis; Carlos Caldas; Fiona M. Watt

doi:10.1016/j.canlet.2009.08.004

Genomic gain of 5p15 leads to over-expression of Misu (NSUN2) in breast cancer

Michaela Frye
, Ilaria Dragoni
, Suet Feung Chin
, Immaculada Spiteri
, Agata Kurowski
, Elena Provenzano
, Andrew Green
, Ian O. Ellis
, Donna Grimmer
, Andrew Teschendorff
, Christos C. Zouboulis
, Carlos Caldas
, Fiona M. Watt^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

80 Scopus citations

Abstract

We have examined expression of the Myc target gene Misu (NSUN2) in breast cancer. There was extensive copy number gain, and increased mRNA and protein levels, of Misu in approximately one third of breast cancer cell lines and primary tumours examined, irrespective of tumour subtype. Genes on 5p15.31-33, where Misu is located, showed evolutionary synteny. siRNA-mediated knockdown of Misu reduced cell number in over half of the cell lines tested, irrespective of estrogen receptor status. We conclude that Misu is up-regulated in a substantial proportion of breast cancers and has therapeutic potential as a drug target.

Original language	English
Pages (from-to)	71-80
Number of pages	10
Journal	Cancer Letters
Volume	289
Issue number	1
DOIs	https://doi.org/10.1016/j.canlet.2009.08.004
State	Published - 1 Mar 2010
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Breast cancer
Gene amplification
Myc
RNA methyltransferase

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10.1016/j.canlet.2009.08.004

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title = "Genomic gain of 5p15 leads to over-expression of Misu (NSUN2) in breast cancer",

abstract = "We have examined expression of the Myc target gene Misu (NSUN2) in breast cancer. There was extensive copy number gain, and increased mRNA and protein levels, of Misu in approximately one third of breast cancer cell lines and primary tumours examined, irrespective of tumour subtype. Genes on 5p15.31-33, where Misu is located, showed evolutionary synteny. siRNA-mediated knockdown of Misu reduced cell number in over half of the cell lines tested, irrespective of estrogen receptor status. We conclude that Misu is up-regulated in a substantial proportion of breast cancers and has therapeutic potential as a drug target.",

keywords = "Breast cancer, Gene amplification, Myc, RNA methyltransferase",

author = "Michaela Frye and Ilaria Dragoni and Chin, \{Suet Feung\} and Immaculada Spiteri and Agata Kurowski and Elena Provenzano and Andrew Green and Ellis, \{Ian O.\} and Donna Grimmer and Andrew Teschendorff and Zouboulis, \{Christos C.\} and Carlos Caldas and Watt, \{Fiona M.\}",

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doi = "10.1016/j.canlet.2009.08.004",

language = "אנגלית",

volume = "289",

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journal = "Cancer Letters",

issn = "0304-3835",

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T1 - Genomic gain of 5p15 leads to over-expression of Misu (NSUN2) in breast cancer

AU - Frye, Michaela

AU - Dragoni, Ilaria

AU - Chin, Suet Feung

AU - Spiteri, Immaculada

AU - Kurowski, Agata

AU - Provenzano, Elena

AU - Green, Andrew

AU - Ellis, Ian O.

AU - Grimmer, Donna

AU - Teschendorff, Andrew

AU - Zouboulis, Christos C.

AU - Caldas, Carlos

AU - Watt, Fiona M.

PY - 2010/3/1

Y1 - 2010/3/1

N2 - We have examined expression of the Myc target gene Misu (NSUN2) in breast cancer. There was extensive copy number gain, and increased mRNA and protein levels, of Misu in approximately one third of breast cancer cell lines and primary tumours examined, irrespective of tumour subtype. Genes on 5p15.31-33, where Misu is located, showed evolutionary synteny. siRNA-mediated knockdown of Misu reduced cell number in over half of the cell lines tested, irrespective of estrogen receptor status. We conclude that Misu is up-regulated in a substantial proportion of breast cancers and has therapeutic potential as a drug target.

AB - We have examined expression of the Myc target gene Misu (NSUN2) in breast cancer. There was extensive copy number gain, and increased mRNA and protein levels, of Misu in approximately one third of breast cancer cell lines and primary tumours examined, irrespective of tumour subtype. Genes on 5p15.31-33, where Misu is located, showed evolutionary synteny. siRNA-mediated knockdown of Misu reduced cell number in over half of the cell lines tested, irrespective of estrogen receptor status. We conclude that Misu is up-regulated in a substantial proportion of breast cancers and has therapeutic potential as a drug target.

KW - Breast cancer

KW - Gene amplification

KW - Myc

KW - RNA methyltransferase

UR - https://www.scopus.com/pages/publications/75949095935

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AN - SCOPUS:75949095935

SN - 0304-3835

VL - 289

SP - 71

EP - 80

JO - Cancer Letters

JF - Cancer Letters

IS - 1

ER -

Genomic gain of 5p15 leads to over-expression of Misu (NSUN2) in breast cancer

Abstract

UN SDGs

Keywords

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