Abstract
CR1 is one of the middle repetitive sequence elements present in the chicken genome. One such repetitive element (GG1-CR1) was found upstream of the chicken CHox E homeobox gene. Sequencing of GG1-CR1 demonstrated that it is one of the longest CR1 elements analyzed. Detailed comparison of all the CR1 sequences published has revealed three subfamilies of CR1 repeats containing various parts of the consensus sequence. We prepared DNA fragments from GG1-CR1 and used them to probe Southern blots and genomic and cDNA library lifts. The results confirm the division of CR1 into three subelements, two of which occur independently in many places in the genome. Northern blot analysis of the CR1 to chicken embryo RNA showed that the CR1 repeat can be part of poly(A)+ transcripts. These results suggest that the CR1 can be transcribed by readthrough from the promoter of the neighboring gene without detrimental effects on the expression of the gene itself. The level of CR1 containing transcripts rises during the first 5 days of embryonic development and then decreases.
| Original language | English |
|---|---|
| Pages (from-to) | 931-939 |
| Number of pages | 9 |
| Journal | Genomics |
| Volume | 10 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 1991 |
Bibliographical note
Funding Information:Avihu Klar for critically reading the manuscript. This work was supported by grants from the United States-Israel Binational Science Foundation (BSF), Jerusalem, Israel (86-00014), and the Fund for Basic Research, administered by The Israel Academy of Sciences and Humanities.