Genomic stability in reprogramming

Daniel Ronen, Nissim Benvenisty*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

47 Scopus citations

Abstract

The genetic stability of induced pluripotent stem (iPS) cells has a significant impact on their potential use in regenerative medicine and basic research. Analysis of the genomic integrity of iPS cells suggests a tendency to develop aberrations ranging from whole chromosome trisomies to single nucleotide mutations. Furthermore, fluctuations in telomere elongation and changes in mitochondrial DNA are also observed. Some mutations may already exist in the founder cells or result from prolonged culturing, however, many of the mutations occur during the reprogramming event. Thus, great care should be given to the initial characterization and subsequent culturing of new iPS cell lines in order to avoid the use of potentially aberrant cells.

Original languageAmerican English
Pages (from-to)444-449
Number of pages6
JournalCurrent Opinion in Genetics and Development
Volume22
Issue number5
DOIs
StatePublished - Oct 2012

Bibliographical note

Funding Information:
The authors would like to thank Tamar Golan-Lev for her assistance with the graphic design, and Uri Ben-David for critical reading of the manuscript. N.B. is the Herbert Cohn Chair in Cancer Research. This research was supported by The Legacy Heritage Biomedical Science Partnership Program of the Israel Science Foundation (grant no. 943/09 ) and by the Centers of Excellence Legacy Heritage Biomedical Science Partnership (grants no. 1801/10 ). D.R. is supported by the Lady Davis Fellowship Trust and the Israel Cancer Research Fund.

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