TY - JOUR
T1 - Genotoxicity monitoring using a 2D-spectroscopic GFP whole cell biosensing system
AU - Bartolome, Amelita J.
AU - Ulber, Roland
AU - Scheper, Thomas
AU - Sagi, Eran
AU - Belkin, Shimshon
PY - 2003/3/1
Y1 - 2003/3/1
N2 - A 2D-spectroscopic genotoxicity biosensing system is presented. This optical sensing system monitors the genotoxicant-induced expression of green fluorescent protein (GFP) in a recombinant Escherichia coli strain. In this organism, GFP synthesis is driven by the recA gene promoter of the bacterial SOS DNA repair system. Using nalidixic acid as a model genotoxicant, we have demonstrated a response range that varies as a function of the excitation wavelength. When GFP fluorescence was monitored at 510 nm, the linear working range to nalidixic acid was within the range of 0.03-0.3 ppm at 390 nm excitation and 0.05-0.3 ppm at 480 nm excitation.
AB - A 2D-spectroscopic genotoxicity biosensing system is presented. This optical sensing system monitors the genotoxicant-induced expression of green fluorescent protein (GFP) in a recombinant Escherichia coli strain. In this organism, GFP synthesis is driven by the recA gene promoter of the bacterial SOS DNA repair system. Using nalidixic acid as a model genotoxicant, we have demonstrated a response range that varies as a function of the excitation wavelength. When GFP fluorescence was monitored at 510 nm, the linear working range to nalidixic acid was within the range of 0.03-0.3 ppm at 390 nm excitation and 0.05-0.3 ppm at 480 nm excitation.
KW - 2D-spectroscopy
KW - Genotoxicity
KW - Green fluorescent protein
KW - Whole cell biosensor
UR - http://www.scopus.com/inward/record.url?scp=0037366371&partnerID=8YFLogxK
U2 - 10.1016/S0925-4005(02)00423-9
DO - 10.1016/S0925-4005(02)00423-9
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AN - SCOPUS:0037366371
SN - 0925-4005
VL - 89
SP - 27
EP - 32
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
IS - 1-2
ER -