TY - JOUR
T1 - Globin mRNA Species Containing Poly(A) Segments of Different Lengths
T2 - Their Functional Stability in Xenopus Oocytes
AU - NUDEL, Uri
AU - SOREQ, Hermona
AU - LITTAUER, Uriel Z.
AU - MARBAIX, Gerard
AU - HUEZ, Georges
AU - LECLERCQ, Madeleine
AU - HUBERT, Evelyne
AU - CHANTRENNE, Hubert
PY - 1976/4
Y1 - 1976/4
N2 - Rabbit globin mRNA species containing poly(A) segments of different lengths were prepared by partial phosphorolysis of mRNA with Escherichia coli polynucleotide phosphorylase. By varying the salt concentration and the time of incubation of the phosphorolysis mixture, as well as performing oligo(dT)‐cellulose chromatography at 22 °C and at 4 °C, globin mRNA preparations containing poly(A) segments of approximately 122, 95, 68, 39, 32, 21, and 16 adenylate residues were obtained. It was found that the functional stability of the mRNA species containing 32 or more adenylate residues after injection into Xenopus laevis oocytes equaled that of the native globin mRNA. On the other hand, the functional stability of mRNA containing an average number of 21 adenylate residues was about 30% of the native mRNA, while that of mRNA containing 16 adenylate residues was as low as nolv(A)‐free globin mRNA.
AB - Rabbit globin mRNA species containing poly(A) segments of different lengths were prepared by partial phosphorolysis of mRNA with Escherichia coli polynucleotide phosphorylase. By varying the salt concentration and the time of incubation of the phosphorolysis mixture, as well as performing oligo(dT)‐cellulose chromatography at 22 °C and at 4 °C, globin mRNA preparations containing poly(A) segments of approximately 122, 95, 68, 39, 32, 21, and 16 adenylate residues were obtained. It was found that the functional stability of the mRNA species containing 32 or more adenylate residues after injection into Xenopus laevis oocytes equaled that of the native globin mRNA. On the other hand, the functional stability of mRNA containing an average number of 21 adenylate residues was about 30% of the native mRNA, while that of mRNA containing 16 adenylate residues was as low as nolv(A)‐free globin mRNA.
UR - http://www.scopus.com/inward/record.url?scp=0017227083&partnerID=8YFLogxK
U2 - 10.1111/j.1432-1033.1976.tb10279.x
DO - 10.1111/j.1432-1033.1976.tb10279.x
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C2 - 776610
AN - SCOPUS:0017227083
SN - 0014-2956
VL - 64
SP - 115
EP - 121
JO - European Journal of Biochemistry
JF - European Journal of Biochemistry
IS - 1
ER -