Globin mRNA Species Containing Poly(A) Segments of Different Lengths: Their Functional Stability in Xenopus Oocytes

Uri NUDEL; Hermona SOREQ; Uriel Z. LITTAUER; Gerard MARBAIX; Georges HUEZ; Madeleine LECLERCQ; Evelyne HUBERT; Hubert CHANTRENNE

doi:10.1111/j.1432-1033.1976.tb10279.x

Globin mRNA Species Containing Poly(A) Segments of Different Lengths: Their Functional Stability in Xenopus Oocytes

Uri NUDEL^*
, Hermona SOREQ
, Uriel Z. LITTAUER
, Gerard MARBAIX
, Georges HUEZ
, Madeleine LECLERCQ
, Evelyne HUBERT
, Hubert CHANTRENNE

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

137 Scopus citations

Abstract

Rabbit globin mRNA species containing poly(A) segments of different lengths were prepared by partial phosphorolysis of mRNA with Escherichia coli polynucleotide phosphorylase. By varying the salt concentration and the time of incubation of the phosphorolysis mixture, as well as performing oligo(dT)‐cellulose chromatography at 22 °C and at 4 °C, globin mRNA preparations containing poly(A) segments of approximately 122, 95, 68, 39, 32, 21, and 16 adenylate residues were obtained. It was found that the functional stability of the mRNA species containing 32 or more adenylate residues after injection into Xenopus laevis oocytes equaled that of the native globin mRNA. On the other hand, the functional stability of mRNA containing an average number of 21 adenylate residues was about 30% of the native mRNA, while that of mRNA containing 16 adenylate residues was as low as nolv(A)‐free globin mRNA.

Original language	English
Pages (from-to)	115-121
Number of pages	7
Journal	European Journal of Biochemistry
Volume	64
Issue number	1
DOIs	https://doi.org/10.1111/j.1432-1033.1976.tb10279.x
State	Published - Apr 1976
Externally published	Yes

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10.1111/j.1432-1033.1976.tb10279.x

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@article{5b84461827a24404aab6bed1f79ae2e9,

title = "Globin mRNA Species Containing Poly(A) Segments of Different Lengths: Their Functional Stability in Xenopus Oocytes",

abstract = "Rabbit globin mRNA species containing poly(A) segments of different lengths were prepared by partial phosphorolysis of mRNA with Escherichia coli polynucleotide phosphorylase. By varying the salt concentration and the time of incubation of the phosphorolysis mixture, as well as performing oligo(dT)‐cellulose chromatography at 22 °C and at 4 °C, globin mRNA preparations containing poly(A) segments of approximately 122, 95, 68, 39, 32, 21, and 16 adenylate residues were obtained. It was found that the functional stability of the mRNA species containing 32 or more adenylate residues after injection into Xenopus laevis oocytes equaled that of the native globin mRNA. On the other hand, the functional stability of mRNA containing an average number of 21 adenylate residues was about 30\% of the native mRNA, while that of mRNA containing 16 adenylate residues was as low as nolv(A)‐free globin mRNA.",

author = "Uri NUDEL and Hermona SOREQ and LITTAUER, \{Uriel Z.\} and Gerard MARBAIX and Georges HUEZ and Madeleine LECLERCQ and Evelyne HUBERT and Hubert CHANTRENNE",

year = "1976",

month = apr,

doi = "10.1111/j.1432-1033.1976.tb10279.x",

language = "אנגלית",

volume = "64",

pages = "115--121",

journal = "European Journal of Biochemistry",

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TY - JOUR

T1 - Globin mRNA Species Containing Poly(A) Segments of Different Lengths

T2 - Their Functional Stability in Xenopus Oocytes

AU - NUDEL, Uri

AU - SOREQ, Hermona

AU - LITTAUER, Uriel Z.

AU - MARBAIX, Gerard

AU - HUEZ, Georges

AU - LECLERCQ, Madeleine

AU - HUBERT, Evelyne

AU - CHANTRENNE, Hubert

PY - 1976/4

Y1 - 1976/4

N2 - Rabbit globin mRNA species containing poly(A) segments of different lengths were prepared by partial phosphorolysis of mRNA with Escherichia coli polynucleotide phosphorylase. By varying the salt concentration and the time of incubation of the phosphorolysis mixture, as well as performing oligo(dT)‐cellulose chromatography at 22 °C and at 4 °C, globin mRNA preparations containing poly(A) segments of approximately 122, 95, 68, 39, 32, 21, and 16 adenylate residues were obtained. It was found that the functional stability of the mRNA species containing 32 or more adenylate residues after injection into Xenopus laevis oocytes equaled that of the native globin mRNA. On the other hand, the functional stability of mRNA containing an average number of 21 adenylate residues was about 30% of the native mRNA, while that of mRNA containing 16 adenylate residues was as low as nolv(A)‐free globin mRNA.

AB - Rabbit globin mRNA species containing poly(A) segments of different lengths were prepared by partial phosphorolysis of mRNA with Escherichia coli polynucleotide phosphorylase. By varying the salt concentration and the time of incubation of the phosphorolysis mixture, as well as performing oligo(dT)‐cellulose chromatography at 22 °C and at 4 °C, globin mRNA preparations containing poly(A) segments of approximately 122, 95, 68, 39, 32, 21, and 16 adenylate residues were obtained. It was found that the functional stability of the mRNA species containing 32 or more adenylate residues after injection into Xenopus laevis oocytes equaled that of the native globin mRNA. On the other hand, the functional stability of mRNA containing an average number of 21 adenylate residues was about 30% of the native mRNA, while that of mRNA containing 16 adenylate residues was as low as nolv(A)‐free globin mRNA.

UR - https://www.scopus.com/pages/publications/0017227083

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DO - 10.1111/j.1432-1033.1976.tb10279.x

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C2 - 776610

AN - SCOPUS:0017227083

SN - 0014-2956

VL - 64

SP - 115

EP - 121

JO - European Journal of Biochemistry

JF - European Journal of Biochemistry

IS - 1

ER -

Globin mRNA Species Containing Poly(A) Segments of Different Lengths: Their Functional Stability in Xenopus Oocytes

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