TY - JOUR
T1 - Glucose release measurements from liposomes with an oxygen electrode
AU - Hertz, Rachel
AU - Barenholz, Yechezkel
PY - 1973/11/30
Y1 - 1973/11/30
N2 - A method for measuring glucose release from liposomes by means of an oxygen electrode is described. The glucose concentration is determined from the initial rates of oxygen reduction catalysed by glucose oxidase. The reduction is directly proportional to either glucose (0.066-3.0 mM) or enzyme concentration (0.3-10 μg). The enzyme does not penetrate lipid bilayers and free glucose can be measured directly. Trapped glucose can be released and measured in the presence of detergents which do not interfere with the assay. By using appropriate calibration curves, glucose concentrations could be determined under extreme conditions of pH (3-9.5), temperature (10-51 °C), and in the presence of various salts and buffers. The application of polarographic methods for studies of the permeability of amino acids and other sugars is discussed.
AB - A method for measuring glucose release from liposomes by means of an oxygen electrode is described. The glucose concentration is determined from the initial rates of oxygen reduction catalysed by glucose oxidase. The reduction is directly proportional to either glucose (0.066-3.0 mM) or enzyme concentration (0.3-10 μg). The enzyme does not penetrate lipid bilayers and free glucose can be measured directly. Trapped glucose can be released and measured in the presence of detergents which do not interfere with the assay. By using appropriate calibration curves, glucose concentrations could be determined under extreme conditions of pH (3-9.5), temperature (10-51 °C), and in the presence of various salts and buffers. The application of polarographic methods for studies of the permeability of amino acids and other sugars is discussed.
UR - http://www.scopus.com/inward/record.url?scp=0015852487&partnerID=8YFLogxK
U2 - 10.1016/0005-2736(73)90279-4
DO - 10.1016/0005-2736(73)90279-4
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C2 - 4762768
AN - SCOPUS:0015852487
SN - 0005-2736
VL - 330
SP - 1
EP - 7
JO - Biochimica et Biophysica Acta - Biomembranes
JF - Biochimica et Biophysica Acta - Biomembranes
IS - 1
ER -