Glucosylation prevents plant defense activation in phloem-feeding insects

Osnat Malka*, Michael L.A.E. Easson, Christian Paetz, Monika Götz, Michael Reichelt, Beate Stein, Katrin Luck, Aleksa Stanišić, Ksenia Juravel, Diego Santos-Garcia, Lilach L. Mondaca, Simon Springate, John Colvin, Stephan Winter, Jonathan Gershenzon, Shai Morin, Daniel G. Vassão*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

The metabolic adaptations by which phloem-feeding insects counteract plant defense compounds are poorly known. Two-component plant defenses, such as glucosinolates, consist of a glucosylated protoxin that is activated by a glycoside hydrolase upon plant damage. Phloem-feeding herbivores are not generally believed to be negatively impacted by two-component defenses due to their slender piercing-sucking mouthparts, which minimize plant damage. However, here we document that glucosinolates are indeed activated during feeding by the whitefly Bemisia tabaci. This phloem feeder was also found to detoxify the majority of the glucosinolates it ingests by the stereoselective addition of glucose moieties, which prevents hydrolytic activation of these defense compounds. Glucosylation of glucosinolates in B. tabaci was accomplished via a transglucosidation mechanism, and two glycoside hydrolase family 13 (GH13) enzymes were shown to catalyze these reactions. This detoxification reaction was also found in a range of other phloem-feeding herbivores. [Figure not available: see fulltext.]

Original languageAmerican English
Pages (from-to)1420-1426
Number of pages7
JournalNature Chemical Biology
Volume16
Issue number12
DOIs
StatePublished - Dec 2020

Bibliographical note

Publisher Copyright:
© 2020, The Author(s), under exclusive licence to Springer Nature America, Inc.

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