Glutamine-stimulated modification and degradation of glutamine synthetase in hepatoma tissue culture cells

Gila Arad*, Aliza Freikopf, Richard G. Kulka

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

39 Scopus citations

Abstract

Effects of glutamine on glutamine synthetase (GS) activity of hepatoma tissue culture (HTC) cells were studied with the aid of a specific goat anti-rat GS serum. Immunodiffusion and immunoelectrophoretic tests show that rat liver GS and HTC cell GS are immunologically similar but not identical. Immunotitrations of HTC cell extracts demonstrate that in cells incubated in high concentrations (5 mM) of glutamine, a cross-reacting form of GS with a decreased enzyme-specific activity accumulates. On prolonged incubation of cells in high glutamine, there is net degradation of GS to form immunologically inactive products. Radioimmunoprecipitation experiments show that glutamine acts by accelerating the degradation of preformed GS.

Original languageEnglish
Pages (from-to)95-101
Number of pages7
JournalCell
Volume8
Issue number1
DOIs
StatePublished - May 1976

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