Glycerol metabolism in the extremely halophilic bacterium Salinibacter ruber

Jonathan Sher, Rahel Elevi, Lily Mana, Aharon Oren*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Growth of Salinibacter ruber, a red, extremely halophilic bacterium phylogenetically affiliated with the Flavobacterium/Cytophaga branch of the domain Bacteria, is stimulated by glycerol. In contrast to glucose consumption, which starts only after more easily degradable substrates present in yeast extract have been depleted, glycerol is consumed during the earliest growth phases. When U-14C-labeled glycerol was added to the culture, up to 25% of the radioactivity was incorporated by the cells. Glycerol kinase activity was detected only in cells grown in the presence of glycerol (up to 90 nmol mg protein-1 min-1). This enzyme functioned over salt concentrations from 0.6 to 2.8 M KCl. No significant activity of NAD-dependent glycerol dehydrogenase was found. It is suggested that Salinibacter may use glycerol as one of its principal substrates in its habitat, the saltern crystallizer ponds.

Original languageAmerican English
Pages (from-to)211-215
Number of pages5
JournalFEMS Microbiology Letters
Volume232
Issue number2
DOIs
StatePublished - 19 Mar 2004

Bibliographical note

Funding Information:
This study was supported by the Israel Science Foundation (Grant 504/03).

Keywords

  • Glycerol
  • Glycerol kinase
  • Halophilic
  • Salinibacter ruber

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