Glycine betaine is the main organic osmotic solute in a stratified microbial community in a hypersaline evaporitic gypsum crust

Aharon Oren*, Rahel Elevi Bardavid, Nesya Kandel, Zeev Aizenshtat, Jan Jehlička

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

We have examined the organic osmotic solutes content within the stratified microbial communities in an evaporitic gypsum crust found in an evaporation pond (~194 g/l total dissolved salts) of the salterns of the Israel Salt Company, Eilat. We extracted intracellular solutes from the upper three pigmented layers of the crust: a yellow-orange layer dominated by unicellular cyanobacteria, a green layer with filamentous cyanobacteria, and a layer colored red-purple by purple sulfur bacteria; dense communities of heterotrophic bacteria were present in all layers. The solutes were analyzed by Raman spectroscopy, 1H and 13C nuclear magnetic resonance, and HPLC. All layers contained glycine betaine as the only detectable osmotic solute; ectoine and other solutes known to be produced by many halophilic and halotolerant prokaryotes were not found. In this first attempt to assess the osmotic solute content within complex natural communities of halophilic microorganisms, the predominant role of glycine betaine as an osmolyte was established. Most heterotrophic bacteria cannot produce glycine betaine but preferentially use it when it is supplied. Presence of glycine betaine produced by the photoautotrophic members of the community, therefore, may relieve the heterotrophs from the need to synthesize other compounds at a high-energy cost.

Original languageAmerican English
Pages (from-to)445-451
Number of pages7
JournalExtremophiles
Volume17
Issue number3
DOIs
StatePublished - May 2013

Bibliographical note

Funding Information:
(to AO and ZA) and by Grant No. P210/10/0467 from the Grant Agency of the Czech Republic and institutional support MSM0021620855 from the Ministry of Education of the Czech Republic (to JJ).

Funding Information:
Acknowledgments We thank Lily Mana for her assistance during the experiments, the Israel Salt Company in Eilat, Israel for allowing access to the salterns, and the staff of the Interuniversity Institute for Marine Sciences of Eilat for logistic support. This study was supported by Grant No. 1103/10 from the Israel Science Foundation

Keywords

  • Compatible solutes
  • Gypsum
  • Halophilic
  • Hypersaline
  • Raman spectroscopy

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