Glycine-rich RNA-binding cofactor RZ1AL is associated with tomato ripening and development

Xindi Li, Yongfang Yang, Ni Zeng, Guiqin Qu, Daqi Fu, Benzhong Zhu, Yunbo Luo, Oren Ostersetzer-Biran, Hongliang Zhu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Tomato ripening is a complex and dynamic process coordinated by many regulatory elements, including plant hormones, transcription factors, and numerous ripening-related RNAs and proteins. Although recent studies have shown that some RNA-binding proteins are involved in the regulation of the ripening process, understanding of how RNA-binding proteins affect fruit ripening is still limited. Here, we report the analysis of a glycine-rich RNA-binding protein, RZ1A-Like (RZ1AL), which plays an important role in tomato ripening, especially fruit coloring. To analyze the functions of RZ1AL in fruit development and ripening, we generated knockout cr-rz1al mutant lines via the CRISPR/Cas9 gene-editing system. Knockout of RZ1AL reduced fruit lycopene content and weight in the cr-rz1al mutant plants. RZ1AL encodes a nucleus-localized protein that is associated with Cajal-related bodies. RNA-seq data demonstrated that the expression levels of genes that encode several key enzymes associated with carotenoid biosynthesis and metabolism were notably downregulated in cr-rz1al fruits. Proteomic analysis revealed that the levels of various ribosomal subunit proteins were reduced. This could affect the translation of ripening-related proteins such as ZDS. Collectively, our findings demonstrate that RZ1AL may participate in the regulation of carotenoid biosynthesis and metabolism and affect tomato development and fruit ripening.

Original languageAmerican English
Article numberuhac134
JournalHorticulture Research
Volume9
DOIs
StatePublished - 2022

Bibliographical note

Funding Information:
This work was funded by grants to H.Z. from the National Natural Science Foundation of China (32061143022 and 31672208) and the 2115 Talent Development Program of China Agricultural University (1061-00109019), and by grants to O.O.B. from the Israeli Science Foundation (ISF; grants 1834/20 and 3254-2020). We greatly appreciate the provision of the binary vector pYLCRISPR/Cas9 system for us by Yaoguang Liu (South China Agriculture University). We also thank Chengguo Duan (Shanghai Center for Plant Stress Biology and Center of Excellence for Molecular Plant Sciences) for providing the 35S:RFPNiFib2 vector.

Funding Information:
This work was funded by grants to H.Z. from the National Natural Science Foundation of China (32061143022 and 31672208) and the 2115 Talent Development Program of China Agricultural University (1061-00109019), and by grants to O.O.B. from the Israeli Science Foundation (ISF; grants 1834/20 and 3254-2020). We greatly appreciate the provision of the binary vector pYLCRISPR/Cas9 system for us by Yaoguang Liu (South China Agriculture University). We also thank Chengguo Duan (Shanghai Center for Plant Stress Biology and Center of Excellence for Molecular Plant Sciences) for providing the 35S:RFP-NiFib2 vector.

Publisher Copyright:
© 2022 The Author(s).

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