Glyphosate inhibits the translocation of green fluorescent protein and sucrose from a transgenic tobacco host to Cuscuta campestris Yunk.

Talia Nadler-Hassar; Alexander Goldshmidt; Baruch Rubin; Shmuel Wolf

doi:10.1007/s00425-004-1288-4

Glyphosate inhibits the translocation of green fluorescent protein and sucrose from a transgenic tobacco host to Cuscuta campestris Yunk.

Talia Nadler-Hassar, Alexander Goldshmidt, Baruch Rubin^*, Shmuel Wolf

^*Corresponding author for this work

Institute of Plant Sciences and Genetics in Agriculture

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The parasitic plant Cuscuta campestris is dependent on its host for water, assimilates and amino acids. It can be controlled by the herbicide glyphosate, which inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), resulting in shikimate accumulation. In this study, C. campestris was parasitic on transgenic tobacco plants expressing green fluorescent protein (GFP) in the phloem. Changes in [¹⁴C]sucrose and GFP accumulation in the parasite were used as indicators of the herbicide's effect on translocation between the host and parasite. Host plants were treated with glyphosate 22 days after sowing. Shikimate accumulation in the parasite 1 day after glyphosate treatment (DAGT) confirmed EPSPS inhibition in C. campestris. No damage was visible in the host plants for the first 3 DAGT, while during that same time, a significant reduction in [¹⁴C]sucrose and GFP accumulation was observed in the parasite. Thus, we propose that the parallel reduction in GFP and sucrose accumulation in C. campestris is a result of a glyphosate effect on the parasite's ability to withdraw assimilates from the host.

Original language	English
Pages (from-to)	790-796
Number of pages	7
Journal	Planta
Volume	219
Issue number	5
DOIs	https://doi.org/10.1007/s00425-004-1288-4
State	Published - Sep 2004

Bibliographical note

Funding Information:
Fig. 7 Shikimate accumulation in C. campestris, and treated host leaf and apex 72 h after glyphosate treatment (five replicates). Means ± SE Acknowledgments This research was supported in part by grant no. TA-MOU-01-CA20-006 from the U.S.–Israel Cooperative Development Research Program, Bureau for Economic Growth, Agriculture, and Trade, U.S. Agency for International Development. The authors are obliged to D.L. Shaner (USDA, AERC, Fort Collins, CO, USA) and S.O. Duke (USDA ARS, NPURU, University, MS, USA) for their critical review of the manuscript.

Keywords

5-Enolpyruvylshikimate-3-phosphate synthase
Cuscuta
Glyphosate
Green fluorescent protein
Sucrose translocation (inhibition)

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10.1007/s00425-004-1288-4

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title = "Glyphosate inhibits the translocation of green fluorescent protein and sucrose from a transgenic tobacco host to Cuscuta campestris Yunk.",

abstract = "The parasitic plant Cuscuta campestris is dependent on its host for water, assimilates and amino acids. It can be controlled by the herbicide glyphosate, which inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), resulting in shikimate accumulation. In this study, C. campestris was parasitic on transgenic tobacco plants expressing green fluorescent protein (GFP) in the phloem. Changes in [14C]sucrose and GFP accumulation in the parasite were used as indicators of the herbicide's effect on translocation between the host and parasite. Host plants were treated with glyphosate 22 days after sowing. Shikimate accumulation in the parasite 1 day after glyphosate treatment (DAGT) confirmed EPSPS inhibition in C. campestris. No damage was visible in the host plants for the first 3 DAGT, while during that same time, a significant reduction in [14C]sucrose and GFP accumulation was observed in the parasite. Thus, we propose that the parallel reduction in GFP and sucrose accumulation in C. campestris is a result of a glyphosate effect on the parasite's ability to withdraw assimilates from the host.",

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author = "Talia Nadler-Hassar and Alexander Goldshmidt and Baruch Rubin and Shmuel Wolf",

note = "Funding Information: Fig. 7 Shikimate accumulation in C. campestris, and treated host leaf and apex 72 h after glyphosate treatment (five replicates). Means ± SE Acknowledgments This research was supported in part by grant no. TA-MOU-01-CA20-006 from the U.S.–Israel Cooperative Development Research Program, Bureau for Economic Growth, Agriculture, and Trade, U.S. Agency for International Development. The authors are obliged to D.L. Shaner (USDA, AERC, Fort Collins, CO, USA) and S.O. Duke (USDA ARS, NPURU, University, MS, USA) for their critical review of the manuscript.",

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AU - Wolf, Shmuel

N1 - Funding Information: Fig. 7 Shikimate accumulation in C. campestris, and treated host leaf and apex 72 h after glyphosate treatment (five replicates). Means ± SE Acknowledgments This research was supported in part by grant no. TA-MOU-01-CA20-006 from the U.S.–Israel Cooperative Development Research Program, Bureau for Economic Growth, Agriculture, and Trade, U.S. Agency for International Development. The authors are obliged to D.L. Shaner (USDA, AERC, Fort Collins, CO, USA) and S.O. Duke (USDA ARS, NPURU, University, MS, USA) for their critical review of the manuscript.

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N2 - The parasitic plant Cuscuta campestris is dependent on its host for water, assimilates and amino acids. It can be controlled by the herbicide glyphosate, which inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), resulting in shikimate accumulation. In this study, C. campestris was parasitic on transgenic tobacco plants expressing green fluorescent protein (GFP) in the phloem. Changes in [14C]sucrose and GFP accumulation in the parasite were used as indicators of the herbicide's effect on translocation between the host and parasite. Host plants were treated with glyphosate 22 days after sowing. Shikimate accumulation in the parasite 1 day after glyphosate treatment (DAGT) confirmed EPSPS inhibition in C. campestris. No damage was visible in the host plants for the first 3 DAGT, while during that same time, a significant reduction in [14C]sucrose and GFP accumulation was observed in the parasite. Thus, we propose that the parallel reduction in GFP and sucrose accumulation in C. campestris is a result of a glyphosate effect on the parasite's ability to withdraw assimilates from the host.

AB - The parasitic plant Cuscuta campestris is dependent on its host for water, assimilates and amino acids. It can be controlled by the herbicide glyphosate, which inhibits 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), resulting in shikimate accumulation. In this study, C. campestris was parasitic on transgenic tobacco plants expressing green fluorescent protein (GFP) in the phloem. Changes in [14C]sucrose and GFP accumulation in the parasite were used as indicators of the herbicide's effect on translocation between the host and parasite. Host plants were treated with glyphosate 22 days after sowing. Shikimate accumulation in the parasite 1 day after glyphosate treatment (DAGT) confirmed EPSPS inhibition in C. campestris. No damage was visible in the host plants for the first 3 DAGT, while during that same time, a significant reduction in [14C]sucrose and GFP accumulation was observed in the parasite. Thus, we propose that the parallel reduction in GFP and sucrose accumulation in C. campestris is a result of a glyphosate effect on the parasite's ability to withdraw assimilates from the host.

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Glyphosate inhibits the translocation of green fluorescent protein and sucrose from a transgenic tobacco host to Cuscuta campestris Yunk.

Abstract

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Keywords

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