Growth hormone inhibits differentiation of avian epiphyseal growth-plate chondrocytes

Efrat Monsonego, Orna Halevy, Arieh Gertler, Shmuel Hurwitz, Mark Pines*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

The effect of chicken growth hormone (cGH) on the proliferation and differentiation of avian growth-plate chondrocyte was evaluated in culture. In culture, addition of ascorbic acid to the culture media caused cell differentiation. Treatment of proliferating chondrocytes with cGH caused a time-dependent increase in collagen type II gene expression together with a decrease in the appearance of osteopontin (OPN) in the medium. In addition, the ascorbic acid-dependent increase in alkaline phosphatase (AP) activity was inhibited by cGH. IGF-I, on the other hand, caused an increase in AP activity in the ascorbic acid-treated chondrocytes. In the presence of ascorbic acid, cGH did not affect collagen type II gene expression or the appearance of OPN in the medium. Proliferation of avian growth-plate chondrocytes, in contrast to mammalian chondrocytes, was not stimulated by GH alone, although the presence of cGH was essential for chondrocyte survival in long-term culture. cGH in combination with epidermal growth factor (EGF) stimulated cell proliferation. These results suggest that GH inhibits differentiation in avian growth-plate chondrocytes, thereby sustaining their proliferative state and maintaining their sensitivity to growth factors such as EGF.

Original languageAmerican English
Pages (from-to)35-42
Number of pages8
JournalMolecular and Cellular Endocrinology
Volume114
Issue number1-2
DOIs
StatePublished - 30 Oct 1995
Externally publishedYes

Bibliographical note

Funding Information:
This study was supportedb y a grant from BARD, the United States-IsraelB inational Agricultural Research and Development Fund (US-2117-92).C on-tribution from the Agricultural Research Organiza-

Keywords

  • Alkaline phosphatase
  • Ascorbic acid
  • Collagen II
  • IGF-I
  • Osteopontin

Fingerprint

Dive into the research topics of 'Growth hormone inhibits differentiation of avian epiphyseal growth-plate chondrocytes'. Together they form a unique fingerprint.

Cite this