Growth of Pseudomonas C on C₁ compounds: continuous culture

Pseudomonas C was grown in continuous culture on methanol, formaldehyde, or formate as sole carbon source. On methanol μ(max) = 0.49/hr and yield constant (Y) = 0.54; on formaldehyde and on unsupplemented media, μ(max) was about 0.2/hr and Y was 0.15, whereas addition of p aminobenzoic acid, folic acid, serine, or glycine to the medium raised Y to about 0.26 to 0.29, and addition of p aminobenzoic acid, folic acid, serine, nicotinamide adenine dinucleotide, and Tween 80 raised the yield to 0.35. On formate and on unsupplemented media, μ(max) = 0.2/hr and Y = 0.02, whereas addition of 0.1 mM p aminobenzoic acid increased μ(max) to about 0.47 and Y to about 0.23. At low cell concentrations or growth rates a beneficial effect of CO₂ was observed. Formaldehyde or formate, when added together with methanol, were utilized simultaneously with the methanol.