H5-type influenza virus hemagglutinin is functionally recognized by the natural killer-activating receptor NKp44

Joanna W. Ho; Oren Hershkovitz; Malik Peiris; Alon Zilka; Ahuva Bar-Ilan; Beatrice Nal; Kid Chu; Mateusz Kudelko; Wing Kam Yiu; Hagit Achdout; Michal Mandelboim; Ralf Altmeyer; Ofer Mandelboim; Roberto Bruzzone; Angel Porgador

doi:10.1128/JVI.02065-07

H5-type influenza virus hemagglutinin is functionally recognized by the natural killer-activating receptor NKp44

Joanna W. Ho
, Oren Hershkovitz
, Malik Peiris
, Alon Zilka
, Ahuva Bar-Ilan
, Beatrice Nal
, Kid Chu
, Mateusz Kudelko
, Wing Kam Yiu
, Hagit Achdout
, Michal Mandelboim
, Ralf Altmeyer
, Ofer Mandelboim
, Roberto Bruzzone
, Angel Porgador^*

^*Corresponding author for this work

Department of Immunology and Cancer Research

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

Antiviral immune defenses involve natural killer (NK) cells. We previously showed that the NK-activating receptor NKp44 is involved in the functional recognition of H1-type influenza virus strains by NK cells. In the present study, we investigated the interaction of NKp44 and the hemagglutinin of a primary influenza virus H5N1 isolate. Here we show that recombinant NKp44 recognizes H5-expressing cells and specifically interacts with soluble H5 hemagglutinin. H5-pseudotyped lentiviral particles bind to NK cells expressing NKp44. Following interaction with target cells expressing H5, pseudotyped lentiviral particles, or membrane-associated H5, NK cells show NKp44-mediated induced activity. These findings indicate that NKp44-H5 interactions induce functional NK activation.

Original language	English
Pages (from-to)	2028-2032
Number of pages	5
Journal	Journal of Virology
Volume	82
Issue number	4
DOIs	https://doi.org/10.1128/JVI.02065-07
State	Published - Feb 2008

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Ho, J. W., Hershkovitz, O., Peiris, M., Zilka, A., Bar-Ilan, A., Nal, B., Chu, K., Kudelko, M., Yiu, W. K., Achdout, H., Mandelboim, M., Altmeyer, R., Mandelboim, O., Bruzzone, R., & Porgador, A. (2008). H5-type influenza virus hemagglutinin is functionally recognized by the natural killer-activating receptor NKp44. Journal of Virology, 82(4), 2028-2032. https://doi.org/10.1128/JVI.02065-07

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title = "H5-type influenza virus hemagglutinin is functionally recognized by the natural killer-activating receptor NKp44",

abstract = "Antiviral immune defenses involve natural killer (NK) cells. We previously showed that the NK-activating receptor NKp44 is involved in the functional recognition of H1-type influenza virus strains by NK cells. In the present study, we investigated the interaction of NKp44 and the hemagglutinin of a primary influenza virus H5N1 isolate. Here we show that recombinant NKp44 recognizes H5-expressing cells and specifically interacts with soluble H5 hemagglutinin. H5-pseudotyped lentiviral particles bind to NK cells expressing NKp44. Following interaction with target cells expressing H5, pseudotyped lentiviral particles, or membrane-associated H5, NK cells show NKp44-mediated induced activity. These findings indicate that NKp44-H5 interactions induce functional NK activation.",

author = "Ho, \{Joanna W.\} and Oren Hershkovitz and Malik Peiris and Alon Zilka and Ahuva Bar-Ilan and Beatrice Nal and Kid Chu and Mateusz Kudelko and Yiu, \{Wing Kam\} and Hagit Achdout and Michal Mandelboim and Ralf Altmeyer and Ofer Mandelboim and Roberto Bruzzone and Angel Porgador",

year = "2008",

month = feb,

doi = "10.1128/JVI.02065-07",

language = "אנגלית",

volume = "82",

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journal = "Journal of Virology",

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}

Ho, JW, Hershkovitz, O, Peiris, M, Zilka, A, Bar-Ilan, A, Nal, B, Chu, K, Kudelko, M, Yiu, WK, Achdout, H, Mandelboim, M, Altmeyer, R, Mandelboim, O, Bruzzone, R & Porgador, A 2008, 'H5-type influenza virus hemagglutinin is functionally recognized by the natural killer-activating receptor NKp44', Journal of Virology, vol. 82, no. 4, pp. 2028-2032. https://doi.org/10.1128/JVI.02065-07

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T1 - H5-type influenza virus hemagglutinin is functionally recognized by the natural killer-activating receptor NKp44

AU - Ho, Joanna W.

AU - Hershkovitz, Oren

AU - Peiris, Malik

AU - Zilka, Alon

AU - Bar-Ilan, Ahuva

AU - Nal, Beatrice

AU - Chu, Kid

AU - Kudelko, Mateusz

AU - Yiu, Wing Kam

AU - Achdout, Hagit

AU - Mandelboim, Michal

AU - Altmeyer, Ralf

AU - Mandelboim, Ofer

AU - Bruzzone, Roberto

AU - Porgador, Angel

PY - 2008/2

Y1 - 2008/2

N2 - Antiviral immune defenses involve natural killer (NK) cells. We previously showed that the NK-activating receptor NKp44 is involved in the functional recognition of H1-type influenza virus strains by NK cells. In the present study, we investigated the interaction of NKp44 and the hemagglutinin of a primary influenza virus H5N1 isolate. Here we show that recombinant NKp44 recognizes H5-expressing cells and specifically interacts with soluble H5 hemagglutinin. H5-pseudotyped lentiviral particles bind to NK cells expressing NKp44. Following interaction with target cells expressing H5, pseudotyped lentiviral particles, or membrane-associated H5, NK cells show NKp44-mediated induced activity. These findings indicate that NKp44-H5 interactions induce functional NK activation.

AB - Antiviral immune defenses involve natural killer (NK) cells. We previously showed that the NK-activating receptor NKp44 is involved in the functional recognition of H1-type influenza virus strains by NK cells. In the present study, we investigated the interaction of NKp44 and the hemagglutinin of a primary influenza virus H5N1 isolate. Here we show that recombinant NKp44 recognizes H5-expressing cells and specifically interacts with soluble H5 hemagglutinin. H5-pseudotyped lentiviral particles bind to NK cells expressing NKp44. Following interaction with target cells expressing H5, pseudotyped lentiviral particles, or membrane-associated H5, NK cells show NKp44-mediated induced activity. These findings indicate that NKp44-H5 interactions induce functional NK activation.

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AN - SCOPUS:38849199239

SN - 0022-538X

VL - 82

SP - 2028

EP - 2032

JO - Journal of Virology

JF - Journal of Virology

IS - 4

ER -

H5-type influenza virus hemagglutinin is functionally recognized by the natural killer-activating receptor NKp44

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