Haladaptatus litoreus sp. nov., an extremely halophilic archaeon from a marine solar saltern, and emended description of the genus Haladaptatus

Heng Lin Cui*, Fang Fang Sun, Xia Gao, Ying Dong, E. Wei Xu, Yu Guang Zhou, Hong Can Liu, Aharon Oren, Pei Jin Zhou

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Two extremely halophilic archaea, strains RO1-28T and RO1-22, were isolated from a marine solar saltern in Jiangsu, China. Both strains required at least 0.05 M Mg2+ and 1.7 M NaCl for growth. They were able to grow over a pH range of 6.0-8.5 and a temperature range of 25-55 °C, with optimal pH of 7.0 and optimal temperature of 37-40 °C. Based on 16S rRNA gene sequence analysis, strains RO1-28T and RO1-22 were closely related to Haladaptatus paucihalophilus, the single species of the genus Haladaptatus, with similarities of 94.0-95.2 %. The major polar lipids of the two strains were phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and three glycolipids chromatographically identical to the glycolipids of Haladaptatus paucihalophilus JCM 13897 T. Both strains RO1-28T and RO1-22 had a DNA G+C content of 54.0 mol% (HPLC). The DNA-DNA hybridization value between the two strains was more than 70% (92 %) and both strains showed low levels of DNA-DNA relatedness (32% and 33 %) with Haladaptatus paucihalophilus JCM 13897T. It was concluded that strains RO1-28T and RO1-22 represent a novel species of the genus Haladaptatus, for which the name Haladaptatus litoreus sp. nov. is proposed. The type strain is RO1-28T (=CGMCC 1.7737T =JCM 15771T).

Original languageAmerican English
Pages (from-to)1085-1089
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume60
Issue number5
DOIs
StatePublished - May 2010

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