Haloanaerobium salsugo sp. nov., a moderately halophilic, anaerobic bacterium from a subterranean brine

V. K. Bhupathiraju, A. Oren, P. K. Sharma, R. S. Tanner, C. R. Woese, M. J. McInerney*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

A strictly anaerobic, moderately halophilic, gram-negative bacterium was isolated from a highly saline oil field brine. The bacterium was a non- spore-forming, nonmotile rod, appearing singly, in pairs, or occasionally as long chains, and measured 0.3 to 0.4 by 2.6 to 4 μm. The bacterium had a specific requirement for NaCl and grew at NaCl concentrations of between 6 and 24%, with optimal growth at 9% NaCl. The isolate grew at temperatures of between 22 and 51°C and pH values of between 5.6 and 8.0. The doubling time in a complex medium containing 10% NaCl was 9 h. Growth was inhibited by chloramphenicol, tetracycline, and penicillin but not by cycloheximide or azide. Fermentable substrates were predominantly carbohydrates. The end products of glucose fermentation were acetate, ethanol, CO2, and H2. The major components of the cellular fatty acids were C(14:0), C(16:0), C(16:1), and C(17:0 cyc) acids. The DNA base composition of the isolate was 34 mol% G+C. Oligonucleotide catalog and sequence analyses of the 16S rRNA showed that strain VS-752(T) was most closely related to Haloanaerobium praevalens GSL(T) (ATCC 33744), the sole member of the genus Haloanaerobium. We propose that strain VS-752 (ATCC 51327) be established as the type strain of a new species, Haloanaerobium salsugo, in the genus Haloanaerobium.

Original languageAmerican English
Pages (from-to)565-572
Number of pages8
JournalInternational Journal of Systematic Bacteriology
Volume44
Issue number3
DOIs
StatePublished - 1994
Externally publishedYes

Fingerprint

Dive into the research topics of 'Haloanaerobium salsugo sp. nov., a moderately halophilic, anaerobic bacterium from a subterranean brine'. Together they form a unique fingerprint.

Cite this