TY - JOUR
T1 - Heat acclimation and heat stress have different effects on cholinergic-induced calcium mobilization
AU - Kaspler, Pavel
AU - Horowitz, Michal
PY - 2001
Y1 - 2001
N2 - There is evidence that the signal transduction array responsible for the secretion of water in evaporative cooling by the submaxillary gland of the rat is subject to heat acclimatory responses. The objectives of the present study were 1) to examine whether heat acclimation affects intracellular Ca2+ mobilization and, in turn, submaxillary glandular responsiveness; 2) to assess whether the acclimatory responses differ from those evoked on heat stress (HS). Experiments were conducted on submaxillary glands of rats acclimated at 34°C for 0, 2 [short-term heat acclimation (STHA)], and 30 [long-term heat acclimation (LTHA)] days. The resting cytosolic calcium concentration ([Ca2+]c) and the carbamylcholine-evoked calcium signal ([Ca2+]s) of dispersed glandular cells were measured using the fluorescent dye fura 2 AM. Inositol-1,4,5-trisphosphate (IP3)-sensitive endoplasmic reticulum Ca2+ stores were determined in permeabilized cells using fura 2 potassium salt. STHA resulted in a drop in both [Ca2+]s and IP3-sensitive Ca2+ stores. On LTHA, the [Ca2+]s amplitude reverted to the preacclimation value, whereas the IP3-sensitive Ca2+ stores remained low. The drop in [Ca2+]s on STHA is in accord with the decreased glandular output (measured by 86Rb efflux) observed during this acclimation phase. However, after LTHA the enhanced glandular output despite reduced [Ca2+]s levels suggests an increased efficiency of cellular secretory mechanisms in that group. Collectively, the alterations in [Ca2+]s support our biphasic acclimation model (Horowitz M, Kaspler P, Marmari Y, and Oron Y. J Appl Physiol 80: 77-85, 1996.). In nonacclimated glands, HS caused an elevation in [Ca2+]s coincidentally with a decrease in the IP3 Ca2+ stores. In contrast, [Ca2+]s in both STHA and LTHA glands was not affected by HS, despite a marked increase in the IP3-sensitive Ca2+ stores in the LTHA glands. The opposing responses to HS and heat acclimation in calcium signaling and stores confirm the specificity of each process.
AB - There is evidence that the signal transduction array responsible for the secretion of water in evaporative cooling by the submaxillary gland of the rat is subject to heat acclimatory responses. The objectives of the present study were 1) to examine whether heat acclimation affects intracellular Ca2+ mobilization and, in turn, submaxillary glandular responsiveness; 2) to assess whether the acclimatory responses differ from those evoked on heat stress (HS). Experiments were conducted on submaxillary glands of rats acclimated at 34°C for 0, 2 [short-term heat acclimation (STHA)], and 30 [long-term heat acclimation (LTHA)] days. The resting cytosolic calcium concentration ([Ca2+]c) and the carbamylcholine-evoked calcium signal ([Ca2+]s) of dispersed glandular cells were measured using the fluorescent dye fura 2 AM. Inositol-1,4,5-trisphosphate (IP3)-sensitive endoplasmic reticulum Ca2+ stores were determined in permeabilized cells using fura 2 potassium salt. STHA resulted in a drop in both [Ca2+]s and IP3-sensitive Ca2+ stores. On LTHA, the [Ca2+]s amplitude reverted to the preacclimation value, whereas the IP3-sensitive Ca2+ stores remained low. The drop in [Ca2+]s on STHA is in accord with the decreased glandular output (measured by 86Rb efflux) observed during this acclimation phase. However, after LTHA the enhanced glandular output despite reduced [Ca2+]s levels suggests an increased efficiency of cellular secretory mechanisms in that group. Collectively, the alterations in [Ca2+]s support our biphasic acclimation model (Horowitz M, Kaspler P, Marmari Y, and Oron Y. J Appl Physiol 80: 77-85, 1996.). In nonacclimated glands, HS caused an elevation in [Ca2+]s coincidentally with a decrease in the IP3 Ca2+ stores. In contrast, [Ca2+]s in both STHA and LTHA glands was not affected by HS, despite a marked increase in the IP3-sensitive Ca2+ stores in the LTHA glands. The opposing responses to HS and heat acclimation in calcium signaling and stores confirm the specificity of each process.
KW - Evaporative cooling
KW - Intracellular calcium
KW - Muscarinic signaling
KW - Salivary gland
UR - http://www.scopus.com/inward/record.url?scp=0034967609&partnerID=8YFLogxK
U2 - 10.1152/ajpregu.2001.280.6.r1688
DO - 10.1152/ajpregu.2001.280.6.r1688
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C2 - 11353672
AN - SCOPUS:0034967609
SN - 0363-6119
VL - 280
SP - R1688-R1696
JO - American Journal of Physiology - Regulatory Integrative and Comparative Physiology
JF - American Journal of Physiology - Regulatory Integrative and Comparative Physiology
IS - 6 49-6
ER -