Helix Stability in Prokaryotic Promoter Regions

Hanah Margalit, Bruce A. Shapiro, Ruth Nussinov, John Owens, Robert L. Jernigan

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Prokaryotic promoters have been extensively studied to relate sequence features to promoter function. Here we examine the relationship between double-helix stability and promoter activity. the double-helix stability is evaluated from sequence data by free energy computation, based on reported values of dinucleotide free energies for strand separation. For a collection of 168 promoters, we find that within a 500-nucleotide span around the transcription initiation site the -10 region is the least stable. There is no correlation between the free energies and the rates of RNA polymerase-promoter open complex formation measured for 25 promoters. We also compare the free energies of 121 promoter mutations across the -35 and -10 consensus regions with the free energies of the corresponding wild-type sequences. These pairwise mutant-wild-type comparisons provide a particularly good test since the examined sequences differ only in one nucleotide so that all other sequence-dependent effects remain the same. About 80% of the mutations in the -10 region that show increased/reduced promoter activity are less/more stable than the wild types. the observed high free energy peak and the mutation data strongly support the conjecture that the instability, or melting properties, of the -10 region plays a significant role in promoter function.

Original languageAmerican English
Pages (from-to)5179-5188
Number of pages10
JournalBiochemistry
Volume27
Issue number14
DOIs
StatePublished - 1 Jul 1988
Externally publishedYes

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