Hemolytic effect of polymeric nanoparticles: Role of albumin

G. Barshtein*, D. Arbell, S. Yedgar

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


Nanoparticles (NP) have drawn increasing interest from many fields in medicine and are a relatively new class of biomedical products. Because there are concerns about the health effects of nanoparticles, it is important to understand how nanoparticles interact specifically with red blood cells (RBC), a central object in the blood circulation. As numerous studies that have examined NP/RBC interaction concentrated on the hemolytic potential of nanoparticles, we describe an investigation of hemolytic activity of polystyrene nanoparticles (PS-NP) in protein free medium and its modulation by albumin. We found that treatment of RBCs with PS-NP induces hemolysis (dose and particle size dependent) in plasma free medium but not in full plasma or in buffer, which contain albumin. Critical albumin concentration is 0.05% wt. According to our results hemolytic effect of nanoparticles is strongly modulated by protein concentration in the medium.

Original languageAmerican English
Article number6086628
Pages (from-to)259-261
Number of pages3
JournalIEEE Transactions on Nanobioscience
Issue number4
StatePublished - Dec 2011

Bibliographical note

Funding Information:
Manuscript received May 17, 2011; accepted October 31, 2011. Date of publication November 22, 2011; date of current version January 20, 2012. The work of S. Yedgar was supported by grants from the Walter and Greta Stiel Chair for Heart Studies and the U.S. National Blood Foundation. The work of G. Barshtein was supported by a grant from the U.S. National Blood Foundation. Asterisk indicates corresponding author. *G. Barshtein is with the Department of Biochemistry, Faculty of Medicine, Hebrew University, 91120 Jerusalem, Israel (e-mail: gregb@cc.huji.ac.il).


  • Erythrocyte
  • hemolysis
  • nanoparticles


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