TY - JOUR
T1 - Hepatitis c virus viremia in scid→bnx mouse chimera
AU - Galun, Eithan
AU - Burakova, Tatjana
AU - Ketzinel, Mali
AU - Lubin, Ido
AU - Shezen, Elias
AU - Kahana, Yuval
AU - Eid, Ahmed
AU - Ilan, Yaron
AU - Rivkind, Avraham
AU - Pizov, Galina
AU - Shouval, Daniel
AU - Reisner, Yair
PY - 1995/7
Y1 - 1995/7
N2 - Chimpanzees are currently the only nonhuman animal model for reproducible propagation of hepatitis C virus (HCV). A chimeric mouse model was used for the induction of hepatitis C viremia, using BNX (beige/nude/X-linked immunodeficient) mice preconditioned by total body irradiation and reconstituted with SCID mouse bone marrow cells. HCV-infected liver fragments from patients with HCV RNA-positive sera were transplanted under the kidney capsule of the chimeric mice. HCV-specific RNA sequences were detected by reverse transcriptase nested polymerase chain reaction (RT-PCR) in serum of âˆ50% of grafted animals. In addition, normal liver specimens were incubated with HCV serum and transplanted into chimeric mice, leading to viremia in âˆ25% of animals. Sequential histologic evaluation of the liver implants, from day 2 to week 14 after transplantation, revealed loss of lobular architecture within the implants. However, viremia persisted for 1050 days after transplantation. These results offer a new HCV model.
AB - Chimpanzees are currently the only nonhuman animal model for reproducible propagation of hepatitis C virus (HCV). A chimeric mouse model was used for the induction of hepatitis C viremia, using BNX (beige/nude/X-linked immunodeficient) mice preconditioned by total body irradiation and reconstituted with SCID mouse bone marrow cells. HCV-infected liver fragments from patients with HCV RNA-positive sera were transplanted under the kidney capsule of the chimeric mice. HCV-specific RNA sequences were detected by reverse transcriptase nested polymerase chain reaction (RT-PCR) in serum of âˆ50% of grafted animals. In addition, normal liver specimens were incubated with HCV serum and transplanted into chimeric mice, leading to viremia in âˆ25% of animals. Sequential histologic evaluation of the liver implants, from day 2 to week 14 after transplantation, revealed loss of lobular architecture within the implants. However, viremia persisted for 1050 days after transplantation. These results offer a new HCV model.
UR - http://www.scopus.com/inward/record.url?scp=0029054973&partnerID=8YFLogxK
U2 - 10.1093/infdis/172.1.25
DO - 10.1093/infdis/172.1.25
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C2 - 7797923
AN - SCOPUS:0029054973
SN - 0022-1899
VL - 172
SP - 25
EP - 30
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 1
ER -
