Herbicide-resistance conferred by expression of a catalytic antibody in Arabidopsis thaliana

Yael Weiss, Avidor Shulman, Irina Ben Shir, Ehud Keinan*, Shmuel Wolf

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Engineering herbicide resistance in crops facilitates control of weed species, particularly those that are closely related to the crop, and may be useful in selecting lines that have undergone multiple transformation events. Here we show that herbicide-resistant plants can be engineered by designing an herbicide and expressing a catalytic antibody that destroys the herbicide in planta. First, we developed a carbamate herbicide that can be catalytically destroyed by the aldolase antibody 38C2. This compound has herbicidal activity on all three plant species tested. Second, the light chain and half of the heavy chain (Fab) of the catalytic antibody were targeted to the endoplasmic reticulum in two classes of Arabidopsis thaliana transformants. Third, the two transgenic plants were crossed to produce an herbicide-resistant F1 hybrid. The in vitro catalytic activity of the protein from F1 hybrids corroborates that catalytic antibodies can be constitutively expressed in transgenic plants, and that they can confer a unique trait.

Original languageAmerican English
Pages (from-to)713-717
Number of pages5
JournalNature Biotechnology
Issue number6
StatePublished - Jun 2006


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