Heritable custom genomic modifications in Caenorhabditis elegans via a CRISPR-Cas9 system

Yonatan B. Tzur, Ari E. Friedland, Saravanapriah Nadarajan, George M. Church, John A. Calarco, Monica P. Colaiácovo

Research output: Contribution to journalComment/debate

112 Scopus citations

Abstract

We adapted the CRISPR-Cas9 system for template-mediated repair of targeted double-strand breaks via homologous recombination in Caenorhabditis elegans, enabling customized and efficient genome editing. This system can be used to create specific insertions, deletions, and base pair changes in the germline of C. elegans.

Original languageEnglish
Pages (from-to)1181-1185
Number of pages5
JournalGenetics
Volume195
Issue number3
DOIs
StatePublished - 2013
Externally publishedYes

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