Heritable custom genomic modifications in Caenorhabditis elegans via a CRISPR-Cas9 system

Yonatan B. Tzur; Ari E. Friedland; Saravanapriah Nadarajan; George M. Church; John A. Calarco; Monica P. Colaiácovo

doi:10.1534/genetics.113.156075

Heritable custom genomic modifications in Caenorhabditis elegans via a CRISPR-Cas9 system

Yonatan B. Tzur
, Ari E. Friedland
, Saravanapriah Nadarajan
, George M. Church
, John A. Calarco
, Monica P. Colaiácovo

Research output: Contribution to journal › Comment/debate

117 Scopus citations

Abstract

We adapted the CRISPR-Cas9 system for template-mediated repair of targeted double-strand breaks via homologous recombination in Caenorhabditis elegans, enabling customized and efficient genome editing. This system can be used to create specific insertions, deletions, and base pair changes in the germline of C. elegans.

Original language	English
Pages (from-to)	1181-1185
Number of pages	5
Journal	Genetics
Volume	195
Issue number	3
DOIs	https://doi.org/10.1534/genetics.113.156075
State	Published - 2013
Externally published	Yes

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10.1534/genetics.113.156075

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AU - Calarco, John A.

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AB - We adapted the CRISPR-Cas9 system for template-mediated repair of targeted double-strand breaks via homologous recombination in Caenorhabditis elegans, enabling customized and efficient genome editing. This system can be used to create specific insertions, deletions, and base pair changes in the germline of C. elegans.

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Heritable custom genomic modifications in Caenorhabditis elegans via a CRISPR-Cas9 system

Abstract

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