Heritable genome editing in C. elegans via a CRISPR-Cas9 system

Ari E. Friedland, Yonatan B. Tzur, Kevin M. Esvelt, Monica P. Colaiácovo, George M. Church*, John A. Calarco

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

721 Scopus citations


We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants.

Original languageAmerican English
Pages (from-to)741-743
Number of pages3
JournalNature Methods
Issue number8
StatePublished - Aug 2013
Externally publishedYes

Bibliographical note

Funding Information:
We thank members of the Caenorhabditis Genetics Center for providing the N2 strain used in our experiments, and B. Stern, A. Murray, A. Saltzman, Joe Calarco and members of the Calarco laboratory for comments on the manuscript. This work was supported by US National Institutes of Health Early Independence Award (1DP5OD009153) and additional support from Harvard University to J.A.C., by National Institutes of Health grant R01GM072551 to M.P.C., and a National Human Genome Research Institute Center of Excellence in Genome Sciences award to G.M.C. A.E.F. is supported by a Ralph Ellison/American Federation for Aging Research postdoctoral fellowship.


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