Heterologous biosynthesis of artemisinin in chrysanthemum morifolium ramat

Aleksey Firsov*, Alexander Pushin, Svetlana Motyleva, Svetlana Pigoleva, Lyubov Shaloiko, Alexander Vainstein, Sergey Dolgov

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Artemisinin-based drugs are the most effective medicine against multidrug-resistant Plas-modium spp., the parasite that causes malaria. To this day, wormwood A. annua L. is the sole commercial source of artemisinin, where it is produced in minor amounts. The artemisinin yield de-pends on numerous poorly regulated agricultural factors and the genetic variability of this non-domesticated plant. This has aroused significant interest in the development of heterologous expression platforms for artemisinin production. Previously, we obtained lines of Chrysanthemum morifolium Ramat. (C. morifolium Ramat.), cvs. White Snowdon and Egyptianka, transformed with artemisinin biosynthesis genes. Here, we report the results of an analysis of artemisinin production in transgenic chrysanthemums. Transcription of heterologous amorpha-4,11-diene monooxygenase and cytochrome P450 reductase genes in transgenic lines was confirmed using high-resolution melt-ing analysis. Artemisinin accumulation was detected using GC-MS in White Snowdon plants, but not in Egyptianka ones, thereby demonstrating the possibility of transplanting active artemisinin biosynthetic pathway into chrysanthemum. Ways of increasing its content in producer plants are discussed.

Original languageAmerican English
Article number75
JournalSeparations
Volume8
Issue number6
DOIs
StatePublished - Jun 2021

Bibliographical note

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Artemisinin
  • Heterologous biosynthesis
  • Malaria
  • Plant biotechnology
  • Plant metabolic engineering
  • Сhrysanthemum morifolium

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