Heterotypic phase separation of Hfq is linked to its roles as an RNA chaperone

Omer Goldberger, Tamar Szoke, Anat Nussbaum-Shochat, Orna Amster-Choder*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Hfq, an Sm-like protein and the major RNA chaperone in E. coli, has been shown to distribute non-uniformly along a helical path under normal growth conditions and to relocate to the cell poles under certain stress conditions. We have previously shown that Hfq relocation to the poles is accompanied by polar accumulation of most small RNAs (sRNAs). Here, we show that Hfq undergoes RNA-dependent phase separation to form cytoplasmic or polar condensates of different density under normal and stress conditions, respectively. Purified Hfq forms droplets in the presence of crowding agents or RNA, indicating that its condensation is via heterotypic interactions. Stress-induced relocation of Hfq condensates and sRNAs to the poles depends on the pole-localizer TmaR. Phase separation of Hfq correlates with its ability to perform its posttranscriptional roles as sRNA-stabilizer and sRNA-mRNA matchmaker. Our study offers a spatiotemporal mechanism for sRNA-mediated regulation in response to environmental changes.

Original languageAmerican English
Article number111881
JournalCell Reports
Volume41
Issue number13
DOIs
StatePublished - 27 Dec 2022

Bibliographical note

Publisher Copyright:
© 2022 The Author(s)

Keywords

  • CP: Molecular biology
  • E. coli
  • Hfq
  • RNA binding proteins
  • TmaR
  • bacterial cell organization
  • biomolecular condensates
  • live-cell microscopy
  • phase separation
  • small RNAs
  • spatiotemporal organization

Fingerprint

Dive into the research topics of 'Heterotypic phase separation of Hfq is linked to its roles as an RNA chaperone'. Together they form a unique fingerprint.

Cite this