Hierarchy in Hfq Chaperon Occupancy of Small RNA Targets Plays a Major Role in Their Regulation

Raya Faigenbaum-Romm, Avichai Reich, Yair E. Gatt, Meshi Barsheshet, Liron Argaman, Hanah Margalit*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Faigenbaum-Romm et al. analyze data of sRNA-target pairs and transcriptome measurements, revealing that only a subset of targets shows expression changes under overexpression of the sRNA. Analyzing various target features, they find that competition among targets over binding the chaperon Hfq plays a major role in the regulatory outcome.

Original languageAmerican English
Pages (from-to)3127-3138.e6
JournalCell Reports
Volume30
Issue number9
DOIs
StatePublished - 3 Mar 2020

Bibliographical note

Funding Information:
This study was supported by the European Research Council (Advanced Grants 322920 and 833598), I-CORE Programs of the Planning and Budgeting Committee and The Israel Science Foundation (grants 1796/12 and 41/1), and the Israel Science Foundation administered by the Israeli Academy for Sciences and Humanities (grant 876/17). Y.E.G. was partially supported by the Hoffman Foundation. We thank T. Bamberger, D. Rabinovich, and M. Kournos for technical help; Y. Altuvia for fruitful discussions; E. Romm for graphical help; and S. Altuvia, A. Peer, S.P. Mizrahi, A. Bar, and M. Nitzan for helpful advice. Experimental Investigation, R.F.-R. M.B. and L.A.; Computational Analysis, R.F.-R. A.R. and Y.E.G.; Writing ? Original Draft, R.F.-R. and H.M.; Writing ? Review & Editing, R.F.-R. L.A. A.R. Y.E.G. M.B. and H.M.; Supervision, H.M. The authors declare no competing interests.

Funding Information:
This study was supported by the European Research Council (Advanced Grants 322920 and 833598 ), I-CORE Programs of the Planning and Budgeting Committee and The Israel Science Foundation (grants 1796/12 and 41/1 ), and the Israel Science Foundation administered by the Israeli Academy for Sciences and Humanities (grant 876/17 ). Y.E.G. was partially supported by the Hoffman Foundation . We thank T. Bamberger, D. Rabinovich, and M. Kournos for technical help; Y. Altuvia for fruitful discussions; E. Romm for graphical help; and S. Altuvia, A. Peer, S.P. Mizrahi, A. Bar, and M. Nitzan for helpful advice.

Publisher Copyright:
© 2020 The Author(s)

Keywords

  • Hfq
  • RIL-seq
  • RNA sequencing
  • posttranscriptional regulation
  • small RNAs

Fingerprint

Dive into the research topics of 'Hierarchy in Hfq Chaperon Occupancy of Small RNA Targets Plays a Major Role in Their Regulation'. Together they form a unique fingerprint.

Cite this