Physical interfaces mediate interactions between multiple types of cells. Despite the importance of such interfaces to the cells’ function, their high-resolution optical imaging has been typically limited due to poor alignment of the interfaces relative to the optical plane of imaging. Here, we present a simple and robust method to align cell-cell interfaces in parallel to the coverslip by adhering the interacting cells to two opposing coverslips and bringing them into contact in a controlled and stable fashion. We demonstrate aberration-free high-resolution imaging of interfaces between live T cells and antigen-presenting cells, known as immune synapses, as an outstanding example. Imaging methods may include multiple diffraction-limited and super-resolution microscopy techniques (e.g., bright-field, confocal, STED, and dSTORM). Thus, our simple and widely compatible approach allows imaging with high- and super-resolution the intricate structure and molecular organization within a variety of cell-cell interfaces.
|Original language||American English|
|Title of host publication||The Immune Synapse|
|Editors||Cosima T. Baldari, Michael L. Dustin|
|Publisher||Humana Press Inc.|
|Number of pages||10|
|ISBN (Print)||978-1-0716-3134-8, 978-1-0716-3137-9|
|State||Published - 2023|
|Name||Methods in Molecular Biology|
Bibliographical notePublisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
- Cell-to-cell interaction
- Diffraction limit
- Immune synapse
- T cells