High- and Super-Resolution Imaging of Cell-Cell Interfaces

Julia Sajman, Eilon Sherman*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Scopus citations

Abstract

Physical interfaces mediate interactions between multiple types of cells. Despite the importance of such interfaces to the cells’ function, their high-resolution optical imaging has been typically limited due to poor alignment of the interfaces relative to the optical plane of imaging. Here, we present a simple and robust method to align cell-cell interfaces in parallel to the coverslip by adhering the interacting cells to two opposing coverslips and bringing them into contact in a controlled and stable fashion. We demonstrate aberration-free high-resolution imaging of interfaces between live T cells and antigen-presenting cells, known as immune synapses, as an outstanding example. Imaging methods may include multiple diffraction-limited and super-resolution microscopy techniques (e.g., bright-field, confocal, STED, and dSTORM). Thus, our simple and widely compatible approach allows imaging with high- and super-resolution the intricate structure and molecular organization within a variety of cell-cell interfaces.

Original languageAmerican English
Title of host publicationThe Immune Synapse
EditorsCosima T. Baldari, Michael L. Dustin
PublisherHumana Press Inc.
Pages149-158
Number of pages10
ISBN (Electronic)978-1-0716-3135-5
ISBN (Print)978-1-0716-3134-8, 978-1-0716-3137-9
DOIs
StatePublished - 2023

Publication series

NameMethods in Molecular Biology
Volume2654
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Bibliographical note

Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

  • Cell-to-cell interaction
  • Diffraction limit
  • Imaging
  • Immune synapse
  • Microcopy
  • Super-resolution
  • T cells

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