TY - JOUR
T1 - High expression of human amelogenin in E. coli.
AU - Deutsch, D.
AU - Chityat, E.
AU - Hekmati, M.
AU - Palmon, A.
AU - Farkash, Y.
AU - Dafni, L.
PY - 1996/11
Y1 - 1996/11
N2 - A human cDNA, encoding for the 175-amino-acid human amelogenin, was prepared by RT PCR from tooth bud mRNA and sub-cloned into pGEX-KG expression plasmid for over-expression in E. coli. The expressed protein was characterized by SDS-PAGE Western blotting, and N-terminal amino acid sequencing.
AB - A human cDNA, encoding for the 175-amino-acid human amelogenin, was prepared by RT PCR from tooth bud mRNA and sub-cloned into pGEX-KG expression plasmid for over-expression in E. coli. The expressed protein was characterized by SDS-PAGE Western blotting, and N-terminal amino acid sequencing.
UR - http://www.scopus.com/inward/record.url?scp=17044453149&partnerID=8YFLogxK
U2 - 10.1177/08959374960100021201
DO - 10.1177/08959374960100021201
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C2 - 9206336
AN - SCOPUS:17044453149
SN - 0895-9374
VL - 10
SP - 187-193; discussion 194
JO - Advances in dental research
JF - Advances in dental research
IS - 2
ER -