High expression of human amelogenin in E. coli.

D. Deutsch*, E. Chityat, M. Hekmati, A. Palmon, Y. Farkash, L. Dafni

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

A human cDNA, encoding for the 175-amino-acid human amelogenin, was prepared by RT PCR from tooth bud mRNA and sub-cloned into pGEX-KG expression plasmid for over-expression in E. coli. The expressed protein was characterized by SDS-PAGE Western blotting, and N-terminal amino acid sequencing.

Original languageAmerican English
Pages (from-to)187-193; discussion 194
JournalAdvances in dental research
Volume10
Issue number2
DOIs
StatePublished - Nov 1996
Externally publishedYes

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