High expression of human amelogenin in E. coli.

D. Deutsch; E. Chityat; M. Hekmati; A. Palmon; Y. Farkash; L. Dafni

doi:10.1177/08959374960100021201

High expression of human amelogenin in E. coli.

D. Deutsch^*
, E. Chityat
, M. Hekmati
, A. Palmon
, Y. Farkash
, L. Dafni

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A human cDNA, encoding for the 175-amino-acid human amelogenin, was prepared by RT PCR from tooth bud mRNA and sub-cloned into pGEX-KG expression plasmid for over-expression in E. coli. The expressed protein was characterized by SDS-PAGE Western blotting, and N-terminal amino acid sequencing.

Original language	English
Pages (from-to)	187-193; discussion 194
Journal	Advances in dental research
Volume	10
Issue number	2
DOIs	https://doi.org/10.1177/08959374960100021201
State	Published - Nov 1996
Externally published	Yes

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title = "High expression of human amelogenin in E. coli.",

abstract = "A human cDNA, encoding for the 175-amino-acid human amelogenin, was prepared by RT PCR from tooth bud mRNA and sub-cloned into pGEX-KG expression plasmid for over-expression in E. coli. The expressed protein was characterized by SDS-PAGE Western blotting, and N-terminal amino acid sequencing.",

author = "D. Deutsch and E. Chityat and M. Hekmati and A. Palmon and Y. Farkash and L. Dafni",

year = "1996",

month = nov,

doi = "10.1177/08959374960100021201",

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AU - Deutsch, D.

AU - Chityat, E.

AU - Hekmati, M.

AU - Palmon, A.

AU - Farkash, Y.

AU - Dafni, L.

PY - 1996/11

Y1 - 1996/11

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AB - A human cDNA, encoding for the 175-amino-acid human amelogenin, was prepared by RT PCR from tooth bud mRNA and sub-cloned into pGEX-KG expression plasmid for over-expression in E. coli. The expressed protein was characterized by SDS-PAGE Western blotting, and N-terminal amino acid sequencing.

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VL - 10

SP - 187-193; discussion 194

JO - Advances in dental research

JF - Advances in dental research

IS - 2

ER -

High expression of human amelogenin in E. coli.

Abstract

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