High oligomycin concentrations augment 6-keto-PGF production in ventricular cardiomyocytes

Arié Pinson*, Yael Zilberman, Régine Tirosh, Victoria Trembovler, Esther Shohami

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Incubation of cultured ventricular cardiomyocytes with high oligomycin concentrations (100 μg/ml), either alone or combined with 2-deoxyglucose (20 mM), led to the rapid depletion of cellular ATP. Inositol (poly)phosphate production decreased, and 6-keto PGF production was increased. In cells depleted of ATP, either by low oligomycin concentrations or by sodium azide, 6-keto PGF was not appreciably increased. There was a 25% rise in the release of fatty acids from the sn-2 position in glycerophospholipids. We suggest that oligomycin at high concentrations causes the release of free arachidonic acid from phospholipids either by non-PIP2-specific PLC and DG lipase or by phospholipase D, phosphatidic acid phosphatase and DG lipase. The effect is unrelated to decreased cellular ATP content.

Original languageEnglish
Pages (from-to)283-288
Number of pages6
JournalBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Volume1211
Issue number3
DOIs
StatePublished - 24 Mar 1994

Keywords

  • Cell injury
  • Cultured heart cell
  • Phospholipase C
  • Prostacyclin production

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