TY - JOUR
T1 - High oligomycin concentrations augment 6-keto-PGF1α production in ventricular cardiomyocytes
AU - Pinson, Arié
AU - Zilberman, Yael
AU - Tirosh, Régine
AU - Trembovler, Victoria
AU - Shohami, Esther
PY - 1994/3/24
Y1 - 1994/3/24
N2 - Incubation of cultured ventricular cardiomyocytes with high oligomycin concentrations (100 μg/ml), either alone or combined with 2-deoxyglucose (20 mM), led to the rapid depletion of cellular ATP. Inositol (poly)phosphate production decreased, and 6-keto PGF1α production was increased. In cells depleted of ATP, either by low oligomycin concentrations or by sodium azide, 6-keto PGF1α was not appreciably increased. There was a 25% rise in the release of fatty acids from the sn-2 position in glycerophospholipids. We suggest that oligomycin at high concentrations causes the release of free arachidonic acid from phospholipids either by non-PIP2-specific PLC and DG lipase or by phospholipase D, phosphatidic acid phosphatase and DG lipase. The effect is unrelated to decreased cellular ATP content.
AB - Incubation of cultured ventricular cardiomyocytes with high oligomycin concentrations (100 μg/ml), either alone or combined with 2-deoxyglucose (20 mM), led to the rapid depletion of cellular ATP. Inositol (poly)phosphate production decreased, and 6-keto PGF1α production was increased. In cells depleted of ATP, either by low oligomycin concentrations or by sodium azide, 6-keto PGF1α was not appreciably increased. There was a 25% rise in the release of fatty acids from the sn-2 position in glycerophospholipids. We suggest that oligomycin at high concentrations causes the release of free arachidonic acid from phospholipids either by non-PIP2-specific PLC and DG lipase or by phospholipase D, phosphatidic acid phosphatase and DG lipase. The effect is unrelated to decreased cellular ATP content.
KW - Cell injury
KW - Cultured heart cell
KW - Phospholipase C
KW - Prostacyclin production
UR - http://www.scopus.com/inward/record.url?scp=0028203754&partnerID=8YFLogxK
U2 - 10.1016/0005-2760(94)90152-X
DO - 10.1016/0005-2760(94)90152-X
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C2 - 8130261
AN - SCOPUS:0028203754
SN - 0005-2760
VL - 1211
SP - 283
EP - 288
JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
IS - 3
ER -