High prevalence of acquired cancer-related mutations in 146 human pluripotent stem cell lines and their differentiated derivatives

Elyad Lezmi, Jonathan Jung, Nissim Benvenisty*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

To survey cancer-related mutations in human pluripotent stem cells and their derivatives, we analyzed >2,200 transcriptomes from 146 independent lines in the NCBI’s Sequence Read Archive. Twenty-two per cent of samples had at least one cancer-related mutation; of these, 64% had TP53 mutations, which conferred a pronounced selective advantage, perturbed target gene expression and altered cellular differentiation. These findings underscore the need for robust surveillance of cancer-related mutations in pluripotent cells, especially in clinical applications.

Original languageAmerican English
JournalNature Biotechnology
Early online date9 Jan 2024
DOIs
StateE-pub ahead of print - 9 Jan 2024

Bibliographical note

Publisher Copyright:
© 2024, The Author(s), under exclusive licence to Springer Nature America, Inc.

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