High-resolution melt curve analysis for identification of single nucleotide mutations in the quinolone resistance gene aac(6′)-Ib-cr

Carlos Hidalgo-Grass*, Jacob Strahilevitz

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

We have developed a simple PCR-based high-resolution melt curve analysis for identification of the quinolone resistance gene aac(6′)-Ib-cr through regions encompassing the two defining single nucleotide mutations. Dissociation curves showed 100% concordance with DNA sequencing, including the identification of a strain where aac(6′)-Ib and aac(6′)-Ib-cr coexist.

Original languageAmerican English
Pages (from-to)3509-3511
Number of pages3
JournalAntimicrobial Agents and Chemotherapy
Volume54
Issue number8
DOIs
StatePublished - 1 Aug 2010
Externally publishedYes

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