TY - JOUR
T1 - High-resolution melt curve analysis for identification of single nucleotide mutations in the quinolone resistance gene aac(6′)-Ib-cr
AU - Hidalgo-Grass, Carlos
AU - Strahilevitz, Jacob
PY - 2010/8/1
Y1 - 2010/8/1
N2 - We have developed a simple PCR-based high-resolution melt curve analysis for identification of the quinolone resistance gene aac(6′)-Ib-cr through regions encompassing the two defining single nucleotide mutations. Dissociation curves showed 100% concordance with DNA sequencing, including the identification of a strain where aac(6′)-Ib and aac(6′)-Ib-cr coexist.
AB - We have developed a simple PCR-based high-resolution melt curve analysis for identification of the quinolone resistance gene aac(6′)-Ib-cr through regions encompassing the two defining single nucleotide mutations. Dissociation curves showed 100% concordance with DNA sequencing, including the identification of a strain where aac(6′)-Ib and aac(6′)-Ib-cr coexist.
UR - http://www.scopus.com/inward/record.url?scp=77955383824&partnerID=8YFLogxK
U2 - 10.1128/AAC.00485-10
DO - 10.1128/AAC.00485-10
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C2 - 20498320
AN - SCOPUS:77955383824
SN - 0066-4804
VL - 54
SP - 3509
EP - 3511
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
IS - 8
ER -