High-resolution melt curve analysis for identification of single nucleotide mutations in the quinolone resistance gene aac(6′)-Ib-cr

Carlos Hidalgo-Grass; Jacob Strahilevitz

doi:10.1128/AAC.00485-10

High-resolution melt curve analysis for identification of single nucleotide mutations in the quinolone resistance gene aac(6′)-Ib-cr

Carlos Hidalgo-Grass^*
, Jacob Strahilevitz

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

We have developed a simple PCR-based high-resolution melt curve analysis for identification of the quinolone resistance gene aac(6′)-Ib-cr through regions encompassing the two defining single nucleotide mutations. Dissociation curves showed 100% concordance with DNA sequencing, including the identification of a strain where aac(6′)-Ib and aac(6′)-Ib-cr coexist.

Original language	English
Pages (from-to)	3509-3511
Number of pages	3
Journal	Antimicrobial Agents and Chemotherapy
Volume	54
Issue number	8
DOIs	https://doi.org/10.1128/AAC.00485-10
State	Published - 1 Aug 2010
Externally published	Yes

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abstract = "We have developed a simple PCR-based high-resolution melt curve analysis for identification of the quinolone resistance gene aac(6′)-Ib-cr through regions encompassing the two defining single nucleotide mutations. Dissociation curves showed 100\% concordance with DNA sequencing, including the identification of a strain where aac(6′)-Ib and aac(6′)-Ib-cr coexist.",

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AB - We have developed a simple PCR-based high-resolution melt curve analysis for identification of the quinolone resistance gene aac(6′)-Ib-cr through regions encompassing the two defining single nucleotide mutations. Dissociation curves showed 100% concordance with DNA sequencing, including the identification of a strain where aac(6′)-Ib and aac(6′)-Ib-cr coexist.

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High-resolution melt curve analysis for identification of single nucleotide mutations in the quinolone resistance gene aac(6′)-Ib-cr

Abstract

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