Nitrogen-vacancy (NV) color centers in diamond have been demonstrated as useful magnetic sensors, in particular for measuring spin fluctuations and achieving high sensitivity and spatial resolution. These abilities can be used to explore various biological and chemical processes, catalyzed by reactive oxygen species (ROS). Here we demonstrate a novel approach to measure and quantify hydroxyl radicals with high spatial resolution, using the fluorescence difference between NV charged states. According to the results, the achieved NV sensitivity is 11±4nMHz, realized in situ without spin labels and localized to a volume of ∼10 picoliters.
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- nitrogen vacancy
- probe intracellular signals