High-Sensitivity, High-Resolution Detection of Reactive Oxygen Species Concentration Using NV Centers

Yoav Ninio, Nir Waiskopf, Idan Meirzada, Yoav Romach, Galya Haim, Shira Yochelis, Uri Banin, Nir Bar-Gill*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Nitrogen-vacancy (NV) color centers in diamond have been demonstrated as useful magnetic sensors, in particular for measuring spin fluctuations and achieving high sensitivity and spatial resolution. These abilities can be used to explore various biological and chemical processes, catalyzed by reactive oxygen species (ROS). Here we demonstrate a novel approach to measure and quantify hydroxyl radicals with high spatial resolution, using the fluorescence difference between NV charged states. According to the results, the achieved NV sensitivity is 11±4nMHz, realized in situ without spin labels and localized to a volume of ∼10 picoliters.

Original languageAmerican English
Pages (from-to)1917-1921
Number of pages5
JournalACS Photonics
Issue number7
StatePublished - 21 Jul 2021

Bibliographical note

Publisher Copyright:
© 2021 American Chemical Society. All rights reserved.


  • fluorescence
  • nitrogen vacancy
  • probe intracellular signals
  • reduction-oxidation


Dive into the research topics of 'High-Sensitivity, High-Resolution Detection of Reactive Oxygen Species Concentration Using NV Centers'. Together they form a unique fingerprint.

Cite this