High-Sensitivity, High-Resolution Detection of Reactive Oxygen Species Concentration Using NV Centers

Yoav Ninio; Nir Waiskopf; Idan Meirzada; Yoav Romach; Galya Haim; Shira Yochelis; Uri Banin; Nir Bar-Gill

doi:10.1021/acsphotonics.1c00431

High-Sensitivity, High-Resolution Detection of Reactive Oxygen Species Concentration Using NV Centers

Yoav Ninio, Nir Waiskopf, Idan Meirzada, Yoav Romach, Galya Haim, Shira Yochelis, Uri Banin, Nir Bar-Gill^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Nitrogen-vacancy (NV) color centers in diamond have been demonstrated as useful magnetic sensors, in particular for measuring spin fluctuations and achieving high sensitivity and spatial resolution. These abilities can be used to explore various biological and chemical processes, catalyzed by reactive oxygen species (ROS). Here we demonstrate a novel approach to measure and quantify hydroxyl radicals with high spatial resolution, using the fluorescence difference between NV charged states. According to the results, the achieved NV sensitivity is 11±4nMHz, realized in situ without spin labels and localized to a volume of ∼10 picoliters.

Original language	English
Pages (from-to)	1917-1921
Number of pages	5
Journal	ACS Photonics
Volume	8
Issue number	7
DOIs	https://doi.org/10.1021/acsphotonics.1c00431
State	Published - 21 Jul 2021

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© 2021 American Chemical Society. All rights reserved.

Keywords

fluorescence
nitrogen vacancy
probe intracellular signals
reduction-oxidation

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10.1021/acsphotonics.1c00431

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title = "High-Sensitivity, High-Resolution Detection of Reactive Oxygen Species Concentration Using NV Centers",

abstract = "Nitrogen-vacancy (NV) color centers in diamond have been demonstrated as useful magnetic sensors, in particular for measuring spin fluctuations and achieving high sensitivity and spatial resolution. These abilities can be used to explore various biological and chemical processes, catalyzed by reactive oxygen species (ROS). Here we demonstrate a novel approach to measure and quantify hydroxyl radicals with high spatial resolution, using the fluorescence difference between NV charged states. According to the results, the achieved NV sensitivity is 11±4nMHz, realized in situ without spin labels and localized to a volume of ∼10 picoliters.",

keywords = "fluorescence, nitrogen vacancy, probe intracellular signals, reduction-oxidation",

author = "Yoav Ninio and Nir Waiskopf and Idan Meirzada and Yoav Romach and Galya Haim and Shira Yochelis and Uri Banin and Nir Bar-Gill",

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AU - Ninio, Yoav

AU - Waiskopf, Nir

AU - Meirzada, Idan

AU - Romach, Yoav

AU - Haim, Galya

AU - Yochelis, Shira

AU - Banin, Uri

AU - Bar-Gill, Nir

PY - 2021/7/21

Y1 - 2021/7/21

N2 - Nitrogen-vacancy (NV) color centers in diamond have been demonstrated as useful magnetic sensors, in particular for measuring spin fluctuations and achieving high sensitivity and spatial resolution. These abilities can be used to explore various biological and chemical processes, catalyzed by reactive oxygen species (ROS). Here we demonstrate a novel approach to measure and quantify hydroxyl radicals with high spatial resolution, using the fluorescence difference between NV charged states. According to the results, the achieved NV sensitivity is 11±4nMHz, realized in situ without spin labels and localized to a volume of ∼10 picoliters.

AB - Nitrogen-vacancy (NV) color centers in diamond have been demonstrated as useful magnetic sensors, in particular for measuring spin fluctuations and achieving high sensitivity and spatial resolution. These abilities can be used to explore various biological and chemical processes, catalyzed by reactive oxygen species (ROS). Here we demonstrate a novel approach to measure and quantify hydroxyl radicals with high spatial resolution, using the fluorescence difference between NV charged states. According to the results, the achieved NV sensitivity is 11±4nMHz, realized in situ without spin labels and localized to a volume of ∼10 picoliters.

KW - fluorescence

KW - nitrogen vacancy

KW - probe intracellular signals

KW - reduction-oxidation

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JO - ACS Photonics

JF - ACS Photonics

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ER -

High-Sensitivity, High-Resolution Detection of Reactive Oxygen Species Concentration Using NV Centers

Abstract

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Keywords

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