High sensitivity of second harmonic generation from styryl dyes to cell membrane potential

Andrew C. Millard; Lei Jin; Aaron Lewis; Leslie M. Loew

High sensitivity of second harmonic generation from styryl dyes to cell membrane potential

Andrew C. Millard, Lei Jin, Aaron Lewis, Leslie M. Loew

The Institute of Applied Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

We present results from the second-harmonic imaging of voltage-clamped cells using membrane-staining dyes. Across a range of excitation wavelengths for different dyes in the ANEP family, we find sensitivities up to 30% per 100mV, significantly better than the optimal ~10% per 100mV of fluorescence.

Original language	English
Title of host publication	Frontiers in Optics, FiO 2003
Publisher	Optica Publishing Group (formerly OSA)
ISBN (Electronic)	1557527598
State	Published - 2003
Event	Frontiers in Optics, FiO 2003 - Tucson, United States Duration: 5 Oct 2003 → …

Publication series

Name	Optics InfoBase Conference Papers
ISSN (Electronic)	2162-2701

Conference

Conference	Frontiers in Optics, FiO 2003
Country/Territory	United States
City	Tucson
Period	5/10/03 → …

Bibliographical note

Publisher Copyright:
© 2000 Optical Society of America

Cite this

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title = "High sensitivity of second harmonic generation from styryl dyes to cell membrane potential",

abstract = "We present results from the second-harmonic imaging of voltage-clamped cells using membrane-staining dyes. Across a range of excitation wavelengths for different dyes in the ANEP family, we find sensitivities up to 30% per 100mV, significantly better than the optimal ~10% per 100mV of fluorescence.",

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AU - Jin, Lei

AU - Lewis, Aaron

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AB - We present results from the second-harmonic imaging of voltage-clamped cells using membrane-staining dyes. Across a range of excitation wavelengths for different dyes in the ANEP family, we find sensitivities up to 30% per 100mV, significantly better than the optimal ~10% per 100mV of fluorescence.

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T3 - Optics InfoBase Conference Papers

BT - Frontiers in Optics, FiO 2003

PB - Optica Publishing Group (formerly OSA)

T2 - Frontiers in Optics, FiO 2003

Y2 - 5 October 2003

ER -

High sensitivity of second harmonic generation from styryl dyes to cell membrane potential

Abstract

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Bibliographical note

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