Higher levels of myelin phospholipids in brains of neuronal α-Synuclein transgenic mice precede myelin loss

Jessica Grigoletto, Katharina Pukaß, Ayelet Gamliel, Dana Davidi, Rachel Katz-Brull, Christiane Richter-Landsberg, Ronit Sharon

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

α-Synuclein is a protein involved in the pathogenesis of synucleinopathies, including Parkinson's disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy (MSA). We investigated the role of neuronal α-Syn in myelin composition and abnormalities. The phospholipid content of purified myelin was determined by 31P NMR in two mouse lines modeling PD, PrP-A53T α-Syn and Thy-1 wt-α-Syn. Significantly higher levels of phospholipids were detected in myelin purified from brains of these α-Syn transgenic mouse models than in control mice. Nevertheless, myelin ultrastructure appeared intact. To further investigate the effect of α-Syn on myelin abnormalities, we systematically analyzed the striatum, a brain region associated with neurodegeneration in PD. An age and disease-dependent loss of myelin basic protein (MBP) signal was detected by immunohistochemistry in striatal striosomes (patches). The age-dependent loss of MBP signal was associated with lower P25α levels in oligodendrocytes. In addition, we found that α-Syn inhibited oligodendrocyte maturation and the formation of membranous sheets in vitro. Based on these results we concluded that neuronal α-Syn is involved in the regulation and/or maintenance of myelin phospholipid. However, axonal hypomyelination in the PD models is evident only in progressive stages of the disease and associated with α-Syn toxicity.

Original languageAmerican English
Pages (from-to)37
Number of pages1
JournalActa neuropathologica communications
Volume5
Issue number1
DOIs
StatePublished - 8 May 2017
Externally publishedYes

Bibliographical note

Funding Information:
JG was supported by a fellowship donated by the Louis Sheinman family and Israel Science Foundation (ISF) grant #182/12. CRL was supported by the Deutsche Forschungsgemeinschaft (DFG Ri 384/16-2). RS was a recipient of a fellowship of the Hanse-Wissenschaftskolleg (HWK), Germany.

Keywords

  • Myelin
  • Parkinson’s disease
  • Phospholipids
  • α-Synuclein

Fingerprint

Dive into the research topics of 'Higher levels of myelin phospholipids in brains of neuronal α-Synuclein transgenic mice precede myelin loss'. Together they form a unique fingerprint.

Cite this