TY - JOUR
T1 - HMGN1 modulates nucleosome occupancy and dnase i hypersensitivity at the CpG island promoters of embryonic stem cells
AU - Deng, Tao
AU - Iris, Zhu Z.
AU - Zhang, Shaofei
AU - Leng, Fenfei
AU - Cherukuri, Srujana
AU - Hansen, Loren
AU - Mariño-Ramírez, Leonardo
AU - Meshorer, Eran
AU - Landsman, David
AU - Bustina, Michael
PY - 2013
Y1 - 2013
N2 - Chromatin structure plays a key role in regulating gene expression and embryonic differentiation; however, the factors that determine the organization of chromatin around regulatory sites are not fully known. Here we show that HMGN1, a nucleosomebinding protein ubiquitously expressed in vertebrate cells, preferentially binds to CpG island-containing promoters and affects the organization of nucleosomes, DNase I hypersensitivity, and the transcriptional profile of mouse embryonic stem cells and neural progenitors. Loss of HMGN1 alters the organization of an unstable nucleosome at transcription start sites, reduces the number of DNase I-hypersensitive sites genome wide, and decreases the number of nestin-positive neural progenitors in the subventricular zone (SVZ) region of mouse brain. Thus, architectural chromatin-binding proteins affect the transcription profile and chromatin structure during embryonic stem cell differentiation
AB - Chromatin structure plays a key role in regulating gene expression and embryonic differentiation; however, the factors that determine the organization of chromatin around regulatory sites are not fully known. Here we show that HMGN1, a nucleosomebinding protein ubiquitously expressed in vertebrate cells, preferentially binds to CpG island-containing promoters and affects the organization of nucleosomes, DNase I hypersensitivity, and the transcriptional profile of mouse embryonic stem cells and neural progenitors. Loss of HMGN1 alters the organization of an unstable nucleosome at transcription start sites, reduces the number of DNase I-hypersensitive sites genome wide, and decreases the number of nestin-positive neural progenitors in the subventricular zone (SVZ) region of mouse brain. Thus, architectural chromatin-binding proteins affect the transcription profile and chromatin structure during embryonic stem cell differentiation
UR - http://www.scopus.com/inward/record.url?scp=84881287170&partnerID=8YFLogxK
U2 - 10.1128/MCB.00435-13
DO - 10.1128/MCB.00435-13
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 23775126
AN - SCOPUS:84881287170
SN - 0270-7306
VL - 33
SP - 3377
EP - 3389
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
IS - 16
ER -