Hormone and forskolin-stimulated cyclic AMP accumulation in human lymphocytes: reliability of longitudinal time measurements

R. P. Ebstein; J. Mintzer; Y. Lipschitz; Z. Shemesh; J. Stessman

doi:10.1007/BF01941546

Hormone and forskolin-stimulated cyclic AMP accumulation in human lymphocytes: reliability of longitudinal time measurements

R. P. Ebstein^*
, J. Mintzer
, Y. Lipschitz
, Z. Shemesh
, J. Stessman

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Reliability of measurement of lymphocyte cyclic AMP synthesis in intact cells was estimated by taking 3 successive blood samples during a one-month period from 11 healthy volunteers. Isoproterenol and prostaglandin E₁-stimulated cyclic AMP accumulation were used to evaluate the activity of these two receptor activities in human lymphocytes. Forskolin-stimulated cyclic AMP accumulation was used to evaluate the activity of the Ns/catalytic subunit. Only for forskolin was significant reliability observed. For isoproterenol and prostaglandin E₁ significant reliability was observed only for male subjects.

Original language	English
Pages (from-to)	838-841
Number of pages	4
Journal	Experientia
Volume	42
Issue number	7
DOIs	https://doi.org/10.1007/BF01941546
State	Published - Jul 1986
Externally published	Yes

Keywords

Cyclic AMP synthesis
forskolin
isoproterenol
measurement in lymphocytes
prostaglandin E

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10.1007/BF01941546

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title = "Hormone and forskolin-stimulated cyclic AMP accumulation in human lymphocytes: reliability of longitudinal time measurements",

abstract = "Reliability of measurement of lymphocyte cyclic AMP synthesis in intact cells was estimated by taking 3 successive blood samples during a one-month period from 11 healthy volunteers. Isoproterenol and prostaglandin E1-stimulated cyclic AMP accumulation were used to evaluate the activity of these two receptor activities in human lymphocytes. Forskolin-stimulated cyclic AMP accumulation was used to evaluate the activity of the Ns/catalytic subunit. Only for forskolin was significant reliability observed. For isoproterenol and prostaglandin E1 significant reliability was observed only for male subjects.",

keywords = "Cyclic AMP synthesis, forskolin, isoproterenol, measurement in lymphocytes, prostaglandin E",

author = "Ebstein, \{R. P.\} and J. Mintzer and Y. Lipschitz and Z. Shemesh and J. Stessman",

year = "1986",

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T1 - Hormone and forskolin-stimulated cyclic AMP accumulation in human lymphocytes

T2 - reliability of longitudinal time measurements

AU - Ebstein, R. P.

AU - Mintzer, J.

AU - Lipschitz, Y.

AU - Shemesh, Z.

AU - Stessman, J.

PY - 1986/7

Y1 - 1986/7

N2 - Reliability of measurement of lymphocyte cyclic AMP synthesis in intact cells was estimated by taking 3 successive blood samples during a one-month period from 11 healthy volunteers. Isoproterenol and prostaglandin E1-stimulated cyclic AMP accumulation were used to evaluate the activity of these two receptor activities in human lymphocytes. Forskolin-stimulated cyclic AMP accumulation was used to evaluate the activity of the Ns/catalytic subunit. Only for forskolin was significant reliability observed. For isoproterenol and prostaglandin E1 significant reliability was observed only for male subjects.

AB - Reliability of measurement of lymphocyte cyclic AMP synthesis in intact cells was estimated by taking 3 successive blood samples during a one-month period from 11 healthy volunteers. Isoproterenol and prostaglandin E1-stimulated cyclic AMP accumulation were used to evaluate the activity of these two receptor activities in human lymphocytes. Forskolin-stimulated cyclic AMP accumulation was used to evaluate the activity of the Ns/catalytic subunit. Only for forskolin was significant reliability observed. For isoproterenol and prostaglandin E1 significant reliability was observed only for male subjects.

KW - Cyclic AMP synthesis

KW - forskolin

KW - isoproterenol

KW - measurement in lymphocytes

KW - prostaglandin E

UR - https://www.scopus.com/pages/publications/0022504493

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AN - SCOPUS:0022504493

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VL - 42

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EP - 841

JO - Experientia

JF - Experientia

IS - 7

ER -

Hormone and forskolin-stimulated cyclic AMP accumulation in human lymphocytes: reliability of longitudinal time measurements

Abstract

Keywords

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