Host-feeding preference of Phlebotomus orientalis (Diptera: Psychodidae) in an endemic focus of visceral leishmaniasis in northern Ethiopia

Araya Gebresilassie; Ibrahim Abbasi; Essayas Aklilu; Solomon Yared; Oscar David Kirstein; Aviad Moncaz; Habte Tekie; Meshesha Balkew; Alon Warburg; Asrat Hailu; Teshome Gebre-Michael

doi:10.1186/s13071-015-0883-5

Host-feeding preference of Phlebotomus orientalis (Diptera: Psychodidae) in an endemic focus of visceral leishmaniasis in northern Ethiopia

Araya Gebresilassie^*, Ibrahim Abbasi, Essayas Aklilu, Solomon Yared, Oscar David Kirstein, Aviad Moncaz, Habte Tekie, Meshesha Balkew, Alon Warburg, Asrat Hailu, Teshome Gebre-Michael

^*Corresponding author for this work

Department of Microbiology and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Background: Blood-feeding behavior studies are important for estimating the efficiency of pathogen transmission and assessing the relative human disease risk. However, in Ethiopia and other parts of East Africa there are large remaining gaps in identifying the feeding habits of Phlebotomus orientalis, the vector of Leishmania donovani. The aim of the study was to determine the blood feeding patterns of P. orientalis in Tahtay Adiyabo district, northern Ethiopia. Methods: For bloodmeal analysis, sandflies were collected from three different villages of Tahtay Adiyabo district using CDC light traps, sticky traps, and pyrethrum spray catches. Bloodmeal of engorged female sandflies was identified using cytochrome (cyt) b-PCR and reverse-line blotting (RLB) and enzyme linked immunosorbent assay (ELISA) assays. Results: Most (637/641) of the females analyzed were P. orientalis. Successful identification of the host from bloodmeals was achieved in 83.03 and 92.1 % using cyt b PCR-RLB and ELISA, respectively. Bloodmeal analysis of P. orientalis females revealed that they have a range of hosts with predominant preference to bovines followed by donkey, human, goat, sheep, dog, and camel. Conclusion: Results obtained from bloodmeal analyses demonstrate that the feeding preference of P. orientalis is mainly zoophilic, which could vary depending on the availability of hosts.

Original language	English
Article number	270
Journal	Parasites and Vectors
Volume	8
Issue number	1
DOIs	https://doi.org/10.1186/s13071-015-0883-5
State	Published - 13 May 2015

Bibliographical note

Funding Information:
We gratefully thank our field assistants Haile Gebremariam and Mehari Naizgi, for their help with collection and processing of sandfly specimens. We are also indebted to the people of Tahtay Adiyabo villagers for their kind co-operation. This study was supported by the Bill and Melinda Gates Foundation Global Health Program (grant number OPPGH5336).

Publisher Copyright:
© 2015 Gebresilassie et al.; licensee BioMed Central.

Keywords

Bloodmeal
Host preference
Phlebotomus orientalis
Tahtay Adiyabo
Visceral leishmaniasis

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1186/s13071-015-0883-5

Cite this

Gebresilassie, A., Abbasi, I., Aklilu, E., Yared, S., Kirstein, O. D., Moncaz, A., Tekie, H., Balkew, M., Warburg, A., Hailu, A., & Gebre-Michael, T. (2015). Host-feeding preference of Phlebotomus orientalis (Diptera: Psychodidae) in an endemic focus of visceral leishmaniasis in northern Ethiopia. Parasites and Vectors, 8(1), Article 270. https://doi.org/10.1186/s13071-015-0883-5

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abstract = "Background: Blood-feeding behavior studies are important for estimating the efficiency of pathogen transmission and assessing the relative human disease risk. However, in Ethiopia and other parts of East Africa there are large remaining gaps in identifying the feeding habits of Phlebotomus orientalis, the vector of Leishmania donovani. The aim of the study was to determine the blood feeding patterns of P. orientalis in Tahtay Adiyabo district, northern Ethiopia. Methods: For bloodmeal analysis, sandflies were collected from three different villages of Tahtay Adiyabo district using CDC light traps, sticky traps, and pyrethrum spray catches. Bloodmeal of engorged female sandflies was identified using cytochrome (cyt) b-PCR and reverse-line blotting (RLB) and enzyme linked immunosorbent assay (ELISA) assays. Results: Most (637/641) of the females analyzed were P. orientalis. Successful identification of the host from bloodmeals was achieved in 83.03 and 92.1 % using cyt b PCR-RLB and ELISA, respectively. Bloodmeal analysis of P. orientalis females revealed that they have a range of hosts with predominant preference to bovines followed by donkey, human, goat, sheep, dog, and camel. Conclusion: Results obtained from bloodmeal analyses demonstrate that the feeding preference of P. orientalis is mainly zoophilic, which could vary depending on the availability of hosts.",

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AU - Gebresilassie, Araya

AU - Abbasi, Ibrahim

AU - Aklilu, Essayas

AU - Yared, Solomon

AU - Kirstein, Oscar David

AU - Moncaz, Aviad

AU - Tekie, Habte

AU - Balkew, Meshesha

AU - Warburg, Alon

AU - Hailu, Asrat

AU - Gebre-Michael, Teshome

PY - 2015/5/13

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N2 - Background: Blood-feeding behavior studies are important for estimating the efficiency of pathogen transmission and assessing the relative human disease risk. However, in Ethiopia and other parts of East Africa there are large remaining gaps in identifying the feeding habits of Phlebotomus orientalis, the vector of Leishmania donovani. The aim of the study was to determine the blood feeding patterns of P. orientalis in Tahtay Adiyabo district, northern Ethiopia. Methods: For bloodmeal analysis, sandflies were collected from three different villages of Tahtay Adiyabo district using CDC light traps, sticky traps, and pyrethrum spray catches. Bloodmeal of engorged female sandflies was identified using cytochrome (cyt) b-PCR and reverse-line blotting (RLB) and enzyme linked immunosorbent assay (ELISA) assays. Results: Most (637/641) of the females analyzed were P. orientalis. Successful identification of the host from bloodmeals was achieved in 83.03 and 92.1 % using cyt b PCR-RLB and ELISA, respectively. Bloodmeal analysis of P. orientalis females revealed that they have a range of hosts with predominant preference to bovines followed by donkey, human, goat, sheep, dog, and camel. Conclusion: Results obtained from bloodmeal analyses demonstrate that the feeding preference of P. orientalis is mainly zoophilic, which could vary depending on the availability of hosts.

AB - Background: Blood-feeding behavior studies are important for estimating the efficiency of pathogen transmission and assessing the relative human disease risk. However, in Ethiopia and other parts of East Africa there are large remaining gaps in identifying the feeding habits of Phlebotomus orientalis, the vector of Leishmania donovani. The aim of the study was to determine the blood feeding patterns of P. orientalis in Tahtay Adiyabo district, northern Ethiopia. Methods: For bloodmeal analysis, sandflies were collected from three different villages of Tahtay Adiyabo district using CDC light traps, sticky traps, and pyrethrum spray catches. Bloodmeal of engorged female sandflies was identified using cytochrome (cyt) b-PCR and reverse-line blotting (RLB) and enzyme linked immunosorbent assay (ELISA) assays. Results: Most (637/641) of the females analyzed were P. orientalis. Successful identification of the host from bloodmeals was achieved in 83.03 and 92.1 % using cyt b PCR-RLB and ELISA, respectively. Bloodmeal analysis of P. orientalis females revealed that they have a range of hosts with predominant preference to bovines followed by donkey, human, goat, sheep, dog, and camel. Conclusion: Results obtained from bloodmeal analyses demonstrate that the feeding preference of P. orientalis is mainly zoophilic, which could vary depending on the availability of hosts.

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Host-feeding preference of Phlebotomus orientalis (Diptera: Psychodidae) in an endemic focus of visceral leishmaniasis in northern Ethiopia

Abstract

Bibliographical note

Keywords

UN SDGs

Access to Document

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