Host specificity and differential pathogenicity of pectobacterium strains from dicot and monocot hosts

Nirmal Khadka; Janak Raj Joshi; Noam Reznik; Nofar Chriker; Adi Nudel; Einat Zelinger; Zohar Kerem; Iris Yedidia

doi:10.3390/microorganisms8101479

Host specificity and differential pathogenicity of pectobacterium strains from dicot and monocot hosts

Nirmal Khadka, Janak Raj Joshi, Noam Reznik, Nofar Chriker, Adi Nudel, Einat Zelinger, Zohar Kerem^*, Iris Yedidia^*

^*Corresponding author for this work

Institute of Biochemistry, Food Science and Nutrition

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Recent phylogenetic studies have transferred certain isolates from monocot plants previously included in the heterogeneous group of Pectobacterium carotovorum (Pc) to a species level termed Pectobacterium aroidearum. The specificity of Pectobacterium associated infections had received less attention, and may be of high scientific and economic importance. Here, we have characterized differential responses of Pectobacterium isolates from potato (WPP14) and calla lily (PC16) on two typical hosts: Brassica oleracea var. capitata (cabbage) a dicot host; and Zantedeschia aethiopica (calla lily) a monocot host. The results revealed clear host specific responses following infection with the two bacterial strains. This was demonstrated by differential production of volatile organic compounds (VOCs) and the expression of plant defense-related genes (pal, PR-1, lox2, ast). A related pattern was observed in bacterial responses to each of the host’s extract, with differential expression of virulence-related determinants and genes associated with quorum-sensing and plant cell wall-degrading enzymes. The differences were associated with each strain’s competence on its respective host.

Original language	American English
Article number	1479
Pages (from-to)	1-22
Number of pages	22
Journal	Microorganisms
Volume	8
Issue number	10
DOIs	https://doi.org/10.3390/microorganisms8101479
State	Published - Oct 2020

Bibliographical note

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

Differential virulence
Host extracts
Host specificity
Pectobacterium
Plant defense

Access to Document

10.3390/microorganisms8101479

Cite this

@article{86493c4dca9f44f7a885c8762d2c37af,

title = "Host specificity and differential pathogenicity of pectobacterium strains from dicot and monocot hosts",

abstract = "Recent phylogenetic studies have transferred certain isolates from monocot plants previously included in the heterogeneous group of Pectobacterium carotovorum (Pc) to a species level termed Pectobacterium aroidearum. The specificity of Pectobacterium associated infections had received less attention, and may be of high scientific and economic importance. Here, we have characterized differential responses of Pectobacterium isolates from potato (WPP14) and calla lily (PC16) on two typical hosts: Brassica oleracea var. capitata (cabbage) a dicot host; and Zantedeschia aethiopica (calla lily) a monocot host. The results revealed clear host specific responses following infection with the two bacterial strains. This was demonstrated by differential production of volatile organic compounds (VOCs) and the expression of plant defense-related genes (pal, PR-1, lox2, ast). A related pattern was observed in bacterial responses to each of the host{\textquoteright}s extract, with differential expression of virulence-related determinants and genes associated with quorum-sensing and plant cell wall-degrading enzymes. The differences were associated with each strain{\textquoteright}s competence on its respective host.",

keywords = "Differential virulence, Host extracts, Host specificity, Pectobacterium, Plant defense",

author = "Nirmal Khadka and Joshi, {Janak Raj} and Noam Reznik and Nofar Chriker and Adi Nudel and Einat Zelinger and Zohar Kerem and Iris Yedidia",

note = "Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2020",

month = oct,

doi = "10.3390/microorganisms8101479",

language = "American English",

volume = "8",

pages = "1--22",

journal = "Microorganisms",

issn = "2076-2607",

publisher = "MDPI AG",

number = "10",

}

TY - JOUR

T1 - Host specificity and differential pathogenicity of pectobacterium strains from dicot and monocot hosts

AU - Khadka, Nirmal

AU - Joshi, Janak Raj

AU - Reznik, Noam

AU - Chriker, Nofar

AU - Nudel, Adi

AU - Zelinger, Einat

AU - Kerem, Zohar

AU - Yedidia, Iris

PY - 2020/10

Y1 - 2020/10

N2 - Recent phylogenetic studies have transferred certain isolates from monocot plants previously included in the heterogeneous group of Pectobacterium carotovorum (Pc) to a species level termed Pectobacterium aroidearum. The specificity of Pectobacterium associated infections had received less attention, and may be of high scientific and economic importance. Here, we have characterized differential responses of Pectobacterium isolates from potato (WPP14) and calla lily (PC16) on two typical hosts: Brassica oleracea var. capitata (cabbage) a dicot host; and Zantedeschia aethiopica (calla lily) a monocot host. The results revealed clear host specific responses following infection with the two bacterial strains. This was demonstrated by differential production of volatile organic compounds (VOCs) and the expression of plant defense-related genes (pal, PR-1, lox2, ast). A related pattern was observed in bacterial responses to each of the host’s extract, with differential expression of virulence-related determinants and genes associated with quorum-sensing and plant cell wall-degrading enzymes. The differences were associated with each strain’s competence on its respective host.

AB - Recent phylogenetic studies have transferred certain isolates from monocot plants previously included in the heterogeneous group of Pectobacterium carotovorum (Pc) to a species level termed Pectobacterium aroidearum. The specificity of Pectobacterium associated infections had received less attention, and may be of high scientific and economic importance. Here, we have characterized differential responses of Pectobacterium isolates from potato (WPP14) and calla lily (PC16) on two typical hosts: Brassica oleracea var. capitata (cabbage) a dicot host; and Zantedeschia aethiopica (calla lily) a monocot host. The results revealed clear host specific responses following infection with the two bacterial strains. This was demonstrated by differential production of volatile organic compounds (VOCs) and the expression of plant defense-related genes (pal, PR-1, lox2, ast). A related pattern was observed in bacterial responses to each of the host’s extract, with differential expression of virulence-related determinants and genes associated with quorum-sensing and plant cell wall-degrading enzymes. The differences were associated with each strain’s competence on its respective host.

KW - Differential virulence

KW - Host extracts

KW - Host specificity

KW - Pectobacterium

KW - Plant defense

UR - http://www.scopus.com/inward/record.url?scp=85091643134&partnerID=8YFLogxK

U2 - 10.3390/microorganisms8101479

DO - 10.3390/microorganisms8101479

M3 - Article

AN - SCOPUS:85091643134

SN - 2076-2607

VL - 8

SP - 1

EP - 22

JO - Microorganisms

JF - Microorganisms

IS - 10

M1 - 1479

ER -

Host specificity and differential pathogenicity of pectobacterium strains from dicot and monocot hosts

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this