Data of protein-protein interactions provide valuable insight into the molecular networks underlying a living cell. However, their accuracy is often questioned, calling for a rigorous assessment of their reliability. The computation offered here provides an intelligible mean to assess directly the rate of true positives in a data set of experimentally determined interacting protein pairs. We show that the reliability of high-throughput yeast two-hybrid assays is about 50%, and that the size of the yeast interactome is estimated to be 10,000-16,600 interactions.
Bibliographical noteFunding Information:
We thank Itay Onn, Nir Friedman and Norman Grover for useful discussions. Joel Sussman is thanked for providing computing facilities to E.S. while in Rehovot. This study was supported by the Israeli Ministry of Science, The Israel Science Foundation (administered by the Israeli Academy of Sciences), and The European Commission (EC grant QLRT-CT-2001-00015-TEMBLOR). E.S. is supported by the Hurvitz Foundation.
- Cellular localization
- Genome-wide analysis
- Protein-protein interaction
- Yeast two-hybrid