HII mesophase and peptide cell-penetrating enhancers for improved transdermal delivery of sodium diclofenac

Marganit Cohen-Avrahami, Abraham Aserin, Nissim Garti*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

77 Scopus citations

Abstract

This study develops a novel transdermal delivery vehicle for the enhanced delivery of sodium diclofenac (Na-DFC). The system utilizes the advantages of reversed hexagonal lyotropic liquid crystals (HIILC), combined with a peptide cell penetration enhancer (CPE), creating together an adaptable system that provides versatile options in the field of transdermal delivery.This enhancer peptide is based on a family of amphipatic peptides that exhibit improved membrane permeability. Franz permeation cell experiments revealed that the peptide enhancer (RALA) improved Na-DFC skin penetration of the liquid crystal 2.2-fold.We studied the structural effects of RALA solubilization on the HII mesophase. RALA acts as a chaotropic agent, interfering in the structure of the water, and causes a measurable swelling of the aqueous cylinders by 5Å.Small angle X-ray scattering (SAXS) and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) measurements reveal enhanced hydration of the glycerol monooleate (GMO) headgroups and a 6.5% increase in the fraction of non-freezable water resulting from RALA incorporation. RALA caused a gradual increase in the GMO effective headgroup area due to the hydration, leading eventually to a transform of the hexagonal structure towards a lamellar one. Circular dichroism and ATR-FTIR measurements showed a conservation of the peptide structure when incorporated into the HII mesophase.The combined HIILC-CPE systems can serve as high potential vehicles for a variety of drugs, as they can easily be modified by varying the composition and temperature, according to the required dose and delivery features.

Original languageEnglish
Pages (from-to)131-138
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume77
Issue number2
DOIs
StatePublished - 2010

Keywords

  • Cell-penetrating peptides
  • Glycerol monooleate
  • Lyotropic liquid crystals
  • RALA
  • Reverse hexagonal mesophase
  • Sodium diclofenac

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