Human embryonic stem cells from aneuploid blastocysts identified by pre-implantation genetic screening

Kavita Narwani, Juan Carlos Biancotti, Tamar Golan-Lev, Nicole Buehler, David Hill, Sagiv Shifman, Nissim Benvenisty, Neta Lavon*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


Human embryonic stem cells are derived from the inner cell mass of pre-implantation embryos. The cells have unlimited proliferation potential and capacity to differentiate into the cells of the three germ layers. Human embryonic stem cells are used to study human embryogenesis and disease modeling and may in the future serve as cells for cell therapy and drug screening. Human embryonic stem cells are usually isolated from surplus normal frozen embryos and were suggested to be isolated from diseased embryos detected by pre-implantation genetic diagnosis. Here we report the isolation of 12 human embryonic stem cell lines and their thorough characterization. The lines were derived from embryos detected to have aneuploidy by pre-implantation genetic screening. Karyotype analysis of these cell lines showed that they are euploid, having 46 chromosomes. Our interpretation is that the euploid cells originated from mosaic embryos, and in vitro selection favored the euploid cells. The undifferentiated cells exhibited long-term proliferation and expressed markers typical for embryonic stem cells such as OCT4, NANOG, and TRA-1-60. The cells manifested pluripotent differentiation both in vivo and in vitro. To further characterize the different lines, we have analyzed their ethnic origin and the family relatedness among them. The above results led us to conclude that the aneuploid mosaic embryos that are destined to be discarded can serve as source for normal euploid human embryonic stem cell lines. These lines represent various ethnic groups; more lines are needed to represent all populations.

Original languageAmerican English
Pages (from-to)309-316
Number of pages8
JournalIn Vitro Cellular and Developmental Biology - Animal
Issue number3-4
StatePublished - Apr 2010

Bibliographical note

Funding Information:
Acknowledgments This work was partially supported by CIRM grant RL1-00636-1: “Generation of Pluripotent Cell Lines from Human Embryos.” We gratefully acknowledge support for this project provided by a grant from the Legacy Heritage Fund of New York.


  • Aneuploidy
  • Cell repository
  • Derivation
  • Human embryonic stem cells
  • Pre-implantation genetic screening


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